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Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders
Hepatitis C virus (HCV) is one of the major risk factors for chronic hepatitis, which may progress to cirrhosis and hepatocellular carcinoma, as well as for type II mixed cryoglobulinemia (MC), which may further evolve into an overt B-cell non-Hodgkin's lymphoma (NHL). It has been previously sh...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839974/ https://www.ncbi.nlm.nih.gov/pubmed/20170491 http://dx.doi.org/10.1186/1479-5876-8-18 |
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author | Buonaguro, Luigi Petrizzo, Annacarmen Tornesello, Marialina Napolitano, Maria Martorelli, Debora Castello, Giuseppe Beneduce, Gerardo De Renzo, Amalia Perrella, Oreste Romagnoli, Luca Sousa, Vitor De Re, Valli Dolcetti, Riccardo Buonaguro, Franco M |
author_facet | Buonaguro, Luigi Petrizzo, Annacarmen Tornesello, Marialina Napolitano, Maria Martorelli, Debora Castello, Giuseppe Beneduce, Gerardo De Renzo, Amalia Perrella, Oreste Romagnoli, Luca Sousa, Vitor De Re, Valli Dolcetti, Riccardo Buonaguro, Franco M |
author_sort | Buonaguro, Luigi |
collection | PubMed |
description | Hepatitis C virus (HCV) is one of the major risk factors for chronic hepatitis, which may progress to cirrhosis and hepatocellular carcinoma, as well as for type II mixed cryoglobulinemia (MC), which may further evolve into an overt B-cell non-Hodgkin's lymphoma (NHL). It has been previously shown that B-cell receptor (BCR) repertoire, expressed by clonal B-cells involved in type II MC as well as in HCV-associated NHL, is constrained to a limited number of variable heavy (VH)- and light (VL)-chain genes. Among these, the VK3-20 light chain idiotype has been selected as a possible target for passive as well as active immunization strategy. In the present study, we describe the results of a multiparametric analysis of the innate and early adaptive immune response after ex vivo stimulation of human immune cells with the VK3-20 protein. This objective has been pursued by implementing high-throughput technologies such as multiparameter flow cytometry and multiplex analysis of cytokines and chemokines. |
format | Text |
id | pubmed-2839974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28399742010-03-17 Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders Buonaguro, Luigi Petrizzo, Annacarmen Tornesello, Marialina Napolitano, Maria Martorelli, Debora Castello, Giuseppe Beneduce, Gerardo De Renzo, Amalia Perrella, Oreste Romagnoli, Luca Sousa, Vitor De Re, Valli Dolcetti, Riccardo Buonaguro, Franco M J Transl Med Research Hepatitis C virus (HCV) is one of the major risk factors for chronic hepatitis, which may progress to cirrhosis and hepatocellular carcinoma, as well as for type II mixed cryoglobulinemia (MC), which may further evolve into an overt B-cell non-Hodgkin's lymphoma (NHL). It has been previously shown that B-cell receptor (BCR) repertoire, expressed by clonal B-cells involved in type II MC as well as in HCV-associated NHL, is constrained to a limited number of variable heavy (VH)- and light (VL)-chain genes. Among these, the VK3-20 light chain idiotype has been selected as a possible target for passive as well as active immunization strategy. In the present study, we describe the results of a multiparametric analysis of the innate and early adaptive immune response after ex vivo stimulation of human immune cells with the VK3-20 protein. This objective has been pursued by implementing high-throughput technologies such as multiparameter flow cytometry and multiplex analysis of cytokines and chemokines. BioMed Central 2010-02-19 /pmc/articles/PMC2839974/ /pubmed/20170491 http://dx.doi.org/10.1186/1479-5876-8-18 Text en Copyright ©2010 Buonaguro et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Buonaguro, Luigi Petrizzo, Annacarmen Tornesello, Marialina Napolitano, Maria Martorelli, Debora Castello, Giuseppe Beneduce, Gerardo De Renzo, Amalia Perrella, Oreste Romagnoli, Luca Sousa, Vitor De Re, Valli Dolcetti, Riccardo Buonaguro, Franco M Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders |
title | Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders |
title_full | Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders |
title_fullStr | Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders |
title_full_unstemmed | Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders |
title_short | Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders |
title_sort | immune signatures in human pbmcs of idiotypic vaccine for hcv-related lymphoproliferative disorders |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839974/ https://www.ncbi.nlm.nih.gov/pubmed/20170491 http://dx.doi.org/10.1186/1479-5876-8-18 |
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