Cargando…

Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders

Hepatitis C virus (HCV) is one of the major risk factors for chronic hepatitis, which may progress to cirrhosis and hepatocellular carcinoma, as well as for type II mixed cryoglobulinemia (MC), which may further evolve into an overt B-cell non-Hodgkin's lymphoma (NHL). It has been previously sh...

Descripción completa

Detalles Bibliográficos
Autores principales: Buonaguro, Luigi, Petrizzo, Annacarmen, Tornesello, Marialina, Napolitano, Maria, Martorelli, Debora, Castello, Giuseppe, Beneduce, Gerardo, De Renzo, Amalia, Perrella, Oreste, Romagnoli, Luca, Sousa, Vitor, De Re, Valli, Dolcetti, Riccardo, Buonaguro, Franco M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839974/
https://www.ncbi.nlm.nih.gov/pubmed/20170491
http://dx.doi.org/10.1186/1479-5876-8-18
_version_ 1782178968312479744
author Buonaguro, Luigi
Petrizzo, Annacarmen
Tornesello, Marialina
Napolitano, Maria
Martorelli, Debora
Castello, Giuseppe
Beneduce, Gerardo
De Renzo, Amalia
Perrella, Oreste
Romagnoli, Luca
Sousa, Vitor
De Re, Valli
Dolcetti, Riccardo
Buonaguro, Franco M
author_facet Buonaguro, Luigi
Petrizzo, Annacarmen
Tornesello, Marialina
Napolitano, Maria
Martorelli, Debora
Castello, Giuseppe
Beneduce, Gerardo
De Renzo, Amalia
Perrella, Oreste
Romagnoli, Luca
Sousa, Vitor
De Re, Valli
Dolcetti, Riccardo
Buonaguro, Franco M
author_sort Buonaguro, Luigi
collection PubMed
description Hepatitis C virus (HCV) is one of the major risk factors for chronic hepatitis, which may progress to cirrhosis and hepatocellular carcinoma, as well as for type II mixed cryoglobulinemia (MC), which may further evolve into an overt B-cell non-Hodgkin's lymphoma (NHL). It has been previously shown that B-cell receptor (BCR) repertoire, expressed by clonal B-cells involved in type II MC as well as in HCV-associated NHL, is constrained to a limited number of variable heavy (VH)- and light (VL)-chain genes. Among these, the VK3-20 light chain idiotype has been selected as a possible target for passive as well as active immunization strategy. In the present study, we describe the results of a multiparametric analysis of the innate and early adaptive immune response after ex vivo stimulation of human immune cells with the VK3-20 protein. This objective has been pursued by implementing high-throughput technologies such as multiparameter flow cytometry and multiplex analysis of cytokines and chemokines.
format Text
id pubmed-2839974
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-28399742010-03-17 Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders Buonaguro, Luigi Petrizzo, Annacarmen Tornesello, Marialina Napolitano, Maria Martorelli, Debora Castello, Giuseppe Beneduce, Gerardo De Renzo, Amalia Perrella, Oreste Romagnoli, Luca Sousa, Vitor De Re, Valli Dolcetti, Riccardo Buonaguro, Franco M J Transl Med Research Hepatitis C virus (HCV) is one of the major risk factors for chronic hepatitis, which may progress to cirrhosis and hepatocellular carcinoma, as well as for type II mixed cryoglobulinemia (MC), which may further evolve into an overt B-cell non-Hodgkin's lymphoma (NHL). It has been previously shown that B-cell receptor (BCR) repertoire, expressed by clonal B-cells involved in type II MC as well as in HCV-associated NHL, is constrained to a limited number of variable heavy (VH)- and light (VL)-chain genes. Among these, the VK3-20 light chain idiotype has been selected as a possible target for passive as well as active immunization strategy. In the present study, we describe the results of a multiparametric analysis of the innate and early adaptive immune response after ex vivo stimulation of human immune cells with the VK3-20 protein. This objective has been pursued by implementing high-throughput technologies such as multiparameter flow cytometry and multiplex analysis of cytokines and chemokines. BioMed Central 2010-02-19 /pmc/articles/PMC2839974/ /pubmed/20170491 http://dx.doi.org/10.1186/1479-5876-8-18 Text en Copyright ©2010 Buonaguro et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Buonaguro, Luigi
Petrizzo, Annacarmen
Tornesello, Marialina
Napolitano, Maria
Martorelli, Debora
Castello, Giuseppe
Beneduce, Gerardo
De Renzo, Amalia
Perrella, Oreste
Romagnoli, Luca
Sousa, Vitor
De Re, Valli
Dolcetti, Riccardo
Buonaguro, Franco M
Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders
title Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders
title_full Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders
title_fullStr Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders
title_full_unstemmed Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders
title_short Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders
title_sort immune signatures in human pbmcs of idiotypic vaccine for hcv-related lymphoproliferative disorders
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839974/
https://www.ncbi.nlm.nih.gov/pubmed/20170491
http://dx.doi.org/10.1186/1479-5876-8-18
work_keys_str_mv AT buonaguroluigi immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders
AT petrizzoannacarmen immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders
AT tornesellomarialina immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders
AT napolitanomaria immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders
AT martorellidebora immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders
AT castellogiuseppe immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders
AT beneducegerardo immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders
AT derenzoamalia immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders
AT perrellaoreste immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders
AT romagnoliluca immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders
AT sousavitor immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders
AT derevalli immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders
AT dolcettiriccardo immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders
AT buonagurofrancom immunesignaturesinhumanpbmcsofidiotypicvaccineforhcvrelatedlymphoproliferativedisorders