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Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso

Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and sat...

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Autores principales: Tiendrébéogo, Fidèle, Lefeuvre, Pierre, Hoareau, Murielle, Villemot, Julie, Konaté, Gnissa, Traoré, Alfred S, Barro, Nicolas, Traoré, Valentin S, Reynaud, Bernard, Traoré, Oumar, Lett, Jean-Michel
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839976/
https://www.ncbi.nlm.nih.gov/pubmed/20178575
http://dx.doi.org/10.1186/1743-422X-7-48
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author Tiendrébéogo, Fidèle
Lefeuvre, Pierre
Hoareau, Murielle
Villemot, Julie
Konaté, Gnissa
Traoré, Alfred S
Barro, Nicolas
Traoré, Valentin S
Reynaud, Bernard
Traoré, Oumar
Lett, Jean-Michel
author_facet Tiendrébéogo, Fidèle
Lefeuvre, Pierre
Hoareau, Murielle
Villemot, Julie
Konaté, Gnissa
Traoré, Alfred S
Barro, Nicolas
Traoré, Valentin S
Reynaud, Bernard
Traoré, Oumar
Lett, Jean-Michel
author_sort Tiendrébéogo, Fidèle
collection PubMed
description Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus (CLCuGV) isolates associated with OLCD, sharing 95 to 99% nucleotide identity, were cloned and sequenced. Six betasatellite and four alphasatellite (DNA-1) molecules were also characterized. The six isolates of betasatellite associated with CLCuGV isolates correspond to Cotton leaf curl Gezira betasatellite (CLCuGB) (88 to 98% nucleotide identity). One isolate of alphasatellite is a variant of Cotton leaf curl Gezira alphasatellite (CLCuGA) (89% nucleotide identity), whereas the three others isolates appear to correspond to a new species of alphasatellite (CLCuGA most similar sequence present 52 to 60% nucleotide identity), provisionally named Okra leaf curl Burkina Faso alphasatellite (OLCBFA). Recombination analysis of the viruses demonstrated the interspecies recombinant origin of all CLCuGV isolates, with parents being close to Hollyhock leaf crumple virus (AY036009) and Tomato leaf curl Diana virus (AM701765). Combined with the presence of satellites DNA, these results highlight the complexity of begomoviruses associated with OLCD.
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spelling pubmed-28399762010-03-17 Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso Tiendrébéogo, Fidèle Lefeuvre, Pierre Hoareau, Murielle Villemot, Julie Konaté, Gnissa Traoré, Alfred S Barro, Nicolas Traoré, Valentin S Reynaud, Bernard Traoré, Oumar Lett, Jean-Michel Virol J Short Report Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus (CLCuGV) isolates associated with OLCD, sharing 95 to 99% nucleotide identity, were cloned and sequenced. Six betasatellite and four alphasatellite (DNA-1) molecules were also characterized. The six isolates of betasatellite associated with CLCuGV isolates correspond to Cotton leaf curl Gezira betasatellite (CLCuGB) (88 to 98% nucleotide identity). One isolate of alphasatellite is a variant of Cotton leaf curl Gezira alphasatellite (CLCuGA) (89% nucleotide identity), whereas the three others isolates appear to correspond to a new species of alphasatellite (CLCuGA most similar sequence present 52 to 60% nucleotide identity), provisionally named Okra leaf curl Burkina Faso alphasatellite (OLCBFA). Recombination analysis of the viruses demonstrated the interspecies recombinant origin of all CLCuGV isolates, with parents being close to Hollyhock leaf crumple virus (AY036009) and Tomato leaf curl Diana virus (AM701765). Combined with the presence of satellites DNA, these results highlight the complexity of begomoviruses associated with OLCD. BioMed Central 2010-02-23 /pmc/articles/PMC2839976/ /pubmed/20178575 http://dx.doi.org/10.1186/1743-422X-7-48 Text en Copyright ©2010 Tiendrébéogo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Tiendrébéogo, Fidèle
Lefeuvre, Pierre
Hoareau, Murielle
Villemot, Julie
Konaté, Gnissa
Traoré, Alfred S
Barro, Nicolas
Traoré, Valentin S
Reynaud, Bernard
Traoré, Oumar
Lett, Jean-Michel
Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso
title Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso
title_full Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso
title_fullStr Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso
title_full_unstemmed Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso
title_short Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso
title_sort molecular diversity of cotton leaf curl gezira virus isolates and their satellite dnas associated with okra leaf curl disease in burkina faso
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839976/
https://www.ncbi.nlm.nih.gov/pubmed/20178575
http://dx.doi.org/10.1186/1743-422X-7-48
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