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Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso
Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and sat...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839976/ https://www.ncbi.nlm.nih.gov/pubmed/20178575 http://dx.doi.org/10.1186/1743-422X-7-48 |
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author | Tiendrébéogo, Fidèle Lefeuvre, Pierre Hoareau, Murielle Villemot, Julie Konaté, Gnissa Traoré, Alfred S Barro, Nicolas Traoré, Valentin S Reynaud, Bernard Traoré, Oumar Lett, Jean-Michel |
author_facet | Tiendrébéogo, Fidèle Lefeuvre, Pierre Hoareau, Murielle Villemot, Julie Konaté, Gnissa Traoré, Alfred S Barro, Nicolas Traoré, Valentin S Reynaud, Bernard Traoré, Oumar Lett, Jean-Michel |
author_sort | Tiendrébéogo, Fidèle |
collection | PubMed |
description | Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus (CLCuGV) isolates associated with OLCD, sharing 95 to 99% nucleotide identity, were cloned and sequenced. Six betasatellite and four alphasatellite (DNA-1) molecules were also characterized. The six isolates of betasatellite associated with CLCuGV isolates correspond to Cotton leaf curl Gezira betasatellite (CLCuGB) (88 to 98% nucleotide identity). One isolate of alphasatellite is a variant of Cotton leaf curl Gezira alphasatellite (CLCuGA) (89% nucleotide identity), whereas the three others isolates appear to correspond to a new species of alphasatellite (CLCuGA most similar sequence present 52 to 60% nucleotide identity), provisionally named Okra leaf curl Burkina Faso alphasatellite (OLCBFA). Recombination analysis of the viruses demonstrated the interspecies recombinant origin of all CLCuGV isolates, with parents being close to Hollyhock leaf crumple virus (AY036009) and Tomato leaf curl Diana virus (AM701765). Combined with the presence of satellites DNA, these results highlight the complexity of begomoviruses associated with OLCD. |
format | Text |
id | pubmed-2839976 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28399762010-03-17 Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso Tiendrébéogo, Fidèle Lefeuvre, Pierre Hoareau, Murielle Villemot, Julie Konaté, Gnissa Traoré, Alfred S Barro, Nicolas Traoré, Valentin S Reynaud, Bernard Traoré, Oumar Lett, Jean-Michel Virol J Short Report Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus (CLCuGV) isolates associated with OLCD, sharing 95 to 99% nucleotide identity, were cloned and sequenced. Six betasatellite and four alphasatellite (DNA-1) molecules were also characterized. The six isolates of betasatellite associated with CLCuGV isolates correspond to Cotton leaf curl Gezira betasatellite (CLCuGB) (88 to 98% nucleotide identity). One isolate of alphasatellite is a variant of Cotton leaf curl Gezira alphasatellite (CLCuGA) (89% nucleotide identity), whereas the three others isolates appear to correspond to a new species of alphasatellite (CLCuGA most similar sequence present 52 to 60% nucleotide identity), provisionally named Okra leaf curl Burkina Faso alphasatellite (OLCBFA). Recombination analysis of the viruses demonstrated the interspecies recombinant origin of all CLCuGV isolates, with parents being close to Hollyhock leaf crumple virus (AY036009) and Tomato leaf curl Diana virus (AM701765). Combined with the presence of satellites DNA, these results highlight the complexity of begomoviruses associated with OLCD. BioMed Central 2010-02-23 /pmc/articles/PMC2839976/ /pubmed/20178575 http://dx.doi.org/10.1186/1743-422X-7-48 Text en Copyright ©2010 Tiendrébéogo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Tiendrébéogo, Fidèle Lefeuvre, Pierre Hoareau, Murielle Villemot, Julie Konaté, Gnissa Traoré, Alfred S Barro, Nicolas Traoré, Valentin S Reynaud, Bernard Traoré, Oumar Lett, Jean-Michel Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso |
title | Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso |
title_full | Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso |
title_fullStr | Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso |
title_full_unstemmed | Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso |
title_short | Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso |
title_sort | molecular diversity of cotton leaf curl gezira virus isolates and their satellite dnas associated with okra leaf curl disease in burkina faso |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839976/ https://www.ncbi.nlm.nih.gov/pubmed/20178575 http://dx.doi.org/10.1186/1743-422X-7-48 |
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