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In vitro antifungal activity of hydroxychavicol isolated from Piper betle L

BACKGROUND: Hydroxychavicol, isolated from the chloroform extraction of the aqueous leaf extract of Piper betle L., (Piperaceae) was investigated for its antifungal activity against 124 strains of selected fungi. The leaves of this plant have been long in use tropical countries for the preparation o...

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Autores principales: Ali, Intzar, Khan, Farrah G, Suri, Krishan A, Gupta, Bishan D, Satti, Naresh K, Dutt, Prabhu, Afrin, Farhat, Qazi, Ghulam N, Khan, Inshad A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2841090/
https://www.ncbi.nlm.nih.gov/pubmed/20128889
http://dx.doi.org/10.1186/1476-0711-9-7
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author Ali, Intzar
Khan, Farrah G
Suri, Krishan A
Gupta, Bishan D
Satti, Naresh K
Dutt, Prabhu
Afrin, Farhat
Qazi, Ghulam N
Khan, Inshad A
author_facet Ali, Intzar
Khan, Farrah G
Suri, Krishan A
Gupta, Bishan D
Satti, Naresh K
Dutt, Prabhu
Afrin, Farhat
Qazi, Ghulam N
Khan, Inshad A
author_sort Ali, Intzar
collection PubMed
description BACKGROUND: Hydroxychavicol, isolated from the chloroform extraction of the aqueous leaf extract of Piper betle L., (Piperaceae) was investigated for its antifungal activity against 124 strains of selected fungi. The leaves of this plant have been long in use tropical countries for the preparation of traditional herbal remedies. METHODS: The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of hydroxychavicol were determined by using broth microdilution method following CLSI guidelines. Time kill curve studies, post-antifungal effects and mutation prevention concentrations were determined against Candida species and Aspergillus species "respectively". Hydroxychavicol was also tested for its potential to inhibit and reduce the formation of Candida albicans biofilms. The membrane permeability was measured by the uptake of propidium iodide. RESULTS: Hydroxychavicol exhibited inhibitory effect on fungal species of clinical significance, with the MICs ranging from 15.62 to 500 μg/ml for yeasts, 125 to 500 μg/ml for Aspergillus species, and 7.81 to 62.5 μg/ml for dermatophytes where as the MFCs were found to be similar or two fold greater than the MICs. There was concentration-dependent killing of Candida albicans and Candida glabrata up to 8 × MIC. Hydroxychavicol also exhibited an extended post antifungal effect of 6.25 to 8.70 h at 4 × MIC for Candida species and suppressed the emergence of mutants of the fungal species tested at 2 × to 8 × MIC concentration. Furthermore, it also inhibited the growth of biofilm generated by C. albicans and reduced the preformed biofilms. There was increased uptake of propidium iodide by C. albicans cells when exposed to hydroxychavicol thus indicating that the membrane disruption could be the probable mode of action of hydroxychavicol. CONCLUSIONS: The antifungal activity exhibited by this compound warrants its use as an antifungal agent particularly for treating topical infections, as well as gargle mouthwash against oral Candida infections.
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spelling pubmed-28410902010-03-18 In vitro antifungal activity of hydroxychavicol isolated from Piper betle L Ali, Intzar Khan, Farrah G Suri, Krishan A Gupta, Bishan D Satti, Naresh K Dutt, Prabhu Afrin, Farhat Qazi, Ghulam N Khan, Inshad A Ann Clin Microbiol Antimicrob Research BACKGROUND: Hydroxychavicol, isolated from the chloroform extraction of the aqueous leaf extract of Piper betle L., (Piperaceae) was investigated for its antifungal activity against 124 strains of selected fungi. The leaves of this plant have been long in use tropical countries for the preparation of traditional herbal remedies. METHODS: The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of hydroxychavicol were determined by using broth microdilution method following CLSI guidelines. Time kill curve studies, post-antifungal effects and mutation prevention concentrations were determined against Candida species and Aspergillus species "respectively". Hydroxychavicol was also tested for its potential to inhibit and reduce the formation of Candida albicans biofilms. The membrane permeability was measured by the uptake of propidium iodide. RESULTS: Hydroxychavicol exhibited inhibitory effect on fungal species of clinical significance, with the MICs ranging from 15.62 to 500 μg/ml for yeasts, 125 to 500 μg/ml for Aspergillus species, and 7.81 to 62.5 μg/ml for dermatophytes where as the MFCs were found to be similar or two fold greater than the MICs. There was concentration-dependent killing of Candida albicans and Candida glabrata up to 8 × MIC. Hydroxychavicol also exhibited an extended post antifungal effect of 6.25 to 8.70 h at 4 × MIC for Candida species and suppressed the emergence of mutants of the fungal species tested at 2 × to 8 × MIC concentration. Furthermore, it also inhibited the growth of biofilm generated by C. albicans and reduced the preformed biofilms. There was increased uptake of propidium iodide by C. albicans cells when exposed to hydroxychavicol thus indicating that the membrane disruption could be the probable mode of action of hydroxychavicol. CONCLUSIONS: The antifungal activity exhibited by this compound warrants its use as an antifungal agent particularly for treating topical infections, as well as gargle mouthwash against oral Candida infections. BioMed Central 2010-02-03 /pmc/articles/PMC2841090/ /pubmed/20128889 http://dx.doi.org/10.1186/1476-0711-9-7 Text en Copyright ©2010 Ali et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Ali, Intzar
Khan, Farrah G
Suri, Krishan A
Gupta, Bishan D
Satti, Naresh K
Dutt, Prabhu
Afrin, Farhat
Qazi, Ghulam N
Khan, Inshad A
In vitro antifungal activity of hydroxychavicol isolated from Piper betle L
title In vitro antifungal activity of hydroxychavicol isolated from Piper betle L
title_full In vitro antifungal activity of hydroxychavicol isolated from Piper betle L
title_fullStr In vitro antifungal activity of hydroxychavicol isolated from Piper betle L
title_full_unstemmed In vitro antifungal activity of hydroxychavicol isolated from Piper betle L
title_short In vitro antifungal activity of hydroxychavicol isolated from Piper betle L
title_sort in vitro antifungal activity of hydroxychavicol isolated from piper betle l
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2841090/
https://www.ncbi.nlm.nih.gov/pubmed/20128889
http://dx.doi.org/10.1186/1476-0711-9-7
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