A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

BACKGROUND: Cultivations for recombinant protein production in shake flasks should provide high cell densities, high protein productivity per cell and good protein quality. The methods described in laboratory handbooks often fail to reach these goals due to oxygen depletion, lack of pH control and t...

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Detalles Bibliográficos
Autores principales: Krause, Mirja, Ukkonen, Kaisa, Haataja, Tatu, Ruottinen, Maria, Glumoff, Tuomo, Neubauer, Antje, Neubauer, Peter, Vasala, Antti
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2841585/
https://www.ncbi.nlm.nih.gov/pubmed/20167131
http://dx.doi.org/10.1186/1475-2859-9-11

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