Cargando…

Evaluation of different expression systems for the heterologous expression of pyranose 2-oxidase from Trametes multicolor in E. coli

The heterologous production of the industrially relevant fungal enzyme pyranose 2-oxidase in the prokaryotic host E. coli was investigated using 3 different expression systems, i.e. the well-studied T7 RNA polymerase based pET21d(+), the L-arabinose inducible pBAD and the pCOLD system. Preliminary e...

Descripción completa

Detalles Bibliográficos
Autores principales:	Spadiut, Oliver, Posch, Gerald, Ludwig, Roland, Haltrich, Dietmar, Peterbauer, Clemens K
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2841586/ https://www.ncbi.nlm.nih.gov/pubmed/20214772 http://dx.doi.org/10.1186/1475-2859-9-14

Ejemplares similares

Oxidation of Phe454 in the Gating Segment Inactivates Trametes multicolor Pyranose Oxidase during Substrate Turnover
por: Halada, Petr, et al.
Publicado: (2016)

Structural Basis for Binding of Fluorinated Glucose and Galactose to Trametes multicolor Pyranose 2-Oxidase Variants with Improved Galactose Conversion
por: Tan, Tien Chye, et al.
Publicado: (2014)

Characterisation of recombinant pyranose oxidase from the cultivated mycorrhizal basidiomycete Lyophyllum shimeji (hon-shimeji)
por: Salaheddin, Clara, et al.
Publicado: (2010)

Engineering Pyranose 2-Oxidase for Modified Oxygen Reactivity
por: Brugger, Dagmar, et al.
Publicado: (2014)

Crystal structures of Phanerochaete chrysosporium pyranose 2-oxidase suggest that the N-terminus acts as a propeptide that assists in homotetramer assembly()
por: Hassan, Noor, et al.
Publicado: (2013)

Characterization of pyranose oxidase variants for bioelectrocatalytic applications
por: Abrera, Annabelle T., et al.
Publicado: (2020)

Versatile Oxidase and Dehydrogenase Activities of Bacterial Pyranose 2-Oxidase Facilitate Redox Cycling with Manganese Peroxidase In Vitro
por: Herzog, Peter L., et al.
Publicado: (2019)

Electrochemical characterization of the pyranose 2-oxidase variant N593C shows a complete loss of the oxidase function with full preservation of substrate (dehydrogenase) activity
por: Brugger, Dagmar, et al.
Publicado: (2016)

Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris
por: Sygmund, Christoph, et al.
Publicado: (2011)

Engineering of Pyranose Dehydrogenase for Increased Oxygen Reactivity
por: Krondorfer, Iris, et al.
Publicado: (2014)

Pyranose Dehydrogenase from Agaricus campestris and Agaricus xanthoderma: Characterization and Applications in Carbohydrate Conversions
por: Staudigl, Petra, et al.
Publicado: (2013)

Biochemical Characterization of Pyranose Oxidase from Streptomyces canus—Towards a Better Understanding of Pyranose Oxidase Homologues in Bacteria
por: Kostelac, Anja, et al.
Publicado: (2022)

Characterization of three pyranose dehydrogenase isoforms from the litter-decomposing basidiomycete Leucoagaricus meleagris (syn. Agaricus meleagris)
por: Graf, Michael M. H., et al.
Publicado: (2016)

The 1.6 Å Crystal Structure of Pyranose Dehydrogenase from Agaricus meleagris Rationalizes Substrate Specificity and Reveals a Flavin Intermediate
por: Tan, Tien Chye, et al.
Publicado: (2013)

Biochemical and mutational analyses of a Trametes pyranose oxidase and comparison of its mutants in breadmaking
por: Li, Mengzhu, et al.
Publicado: (2018)

Localization of Pyranose 2-Oxidase from Kitasatospora aureofaciens: A Step Closer to Elucidate a Biological Role
por: Virginia, Ludovika Jessica, et al.
Publicado: (2023)

Agaricus meleagris pyranose dehydrogenase: Influence of covalent FAD linkage on catalysis and stability
por: Krondorfer, Iris, et al.
Publicado: (2014)

Reaction of pyranose dehydrogenase from Agaricus meleagris with its carbohydrate substrates
por: Graf, Michael M.H., et al.
Publicado: (2015)

Expression, purification, and characterization of galactose oxidase of Fusarium sambucinum in E. coli
por: Paukner, Regina, et al.
Publicado: (2015)

Heterologous expression of a recombinant lactobacillal β-galactosidase in Lactobacillus plantarum: effect of different parameters on the sakacin P-based expression system
por: Nguyen, Tien-Thanh, et al.
Publicado: (2015)

Further Insights into the Catalytical Properties of Deglycosylated Pyranose Dehydrogenase from Agaricus meleagris Recombinantly Expressed in Pichia pastoris
por: Yakovleva, Maria E., et al.
Publicado: (2013)

Galactose Oxidase from Fusarium oxysporum - Expression in E. coli and P. pastoris and Biochemical Characterization
por: Paukner, Regina, et al.
Publicado: (2014)

Heterologous overexpression of Glomerella cingulata FAD-dependent glucose dehydrogenase in Escherichia coli and Pichia pastoris
por: Sygmund, Christoph, et al.
Publicado: (2011)

Molecular dynamics simulations give insight into d-glucose dioxidation at C2 and C3 by Agaricus meleagris pyranose dehydrogenase
por: Graf, Michael M. H., et al.
Publicado: (2013)

Xenobiotic Compounds Degradation by Heterologous Expression of a Trametes sanguineus Laccase in Trichoderma atroviride
por: Balcázar-López, Edgar, et al.
Publicado: (2016)

Pyranose Dehydrogenase Ligand Promiscuity: A Generalized Approach to Simulate Monosaccharide Solvation, Binding, and Product Formation
por: Graf, Michael M. H., et al.
Publicado: (2014)

l-Arabinose Isomerase and d-Xylose Isomerase from Lactobacillus reuteri: Characterization, Coexpression in the Food Grade Host Lactobacillus plantarum, and Application in the Conversion of d-Galactose and d-Glucose
por: Staudigl, Petra, et al.
Publicado: (2014)

Heterologous expression of lcc1 gene from Trametes trogii in Pichia pastoris and characterization of the recombinant enzyme
por: Colao, Maria Chiara, et al.
Publicado: (2006)

Analysis and Reconstitution of the Menaquinone Biosynthesis Pathway in Lactiplantibacillus plantarum and Lentilactibacillus buchneri
por: Watthanasakphuban, Nisit, et al.
Publicado: (2021)

A comparative summary of expression systems for the recombinant production of galactose oxidase
por: Spadiut, Oliver, et al.
Publicado: (2010)

Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus
por: Rocha, Saul N, et al.
Publicado: (2010)

Synthesis of 3-Keto-levoglucosan Using Pyranose Oxidase and Its Spontaneous Decomposition via β-Elimination
por: Kitaoka, Motomitsu
Publicado: (2017)

Quantitative transcript analysis of the inducible expression system pSIP: comparison of the overexpression of Lactobacillus spp. β-galactosidases in Lactobacillus plantarum
por: Nguyen, Tien-Thanh, et al.
Publicado: (2011)

Constitutive expression and cell-surface display of a bacterial β-mannanase in Lactobacillus plantarum
por: Nguyen, Hoang-Minh, et al.
Publicado: (2019)

Updated restraint dictionaries for carbohydrates in the pyranose form
por: Atanasova, Mihaela, et al.
Publicado: (2022)

The price of flexibility – a case study on septanoses as pyranose mimetics
por: Sager, Christoph P., et al.
Publicado: (2017)

Factors involved in heterologous expression of proteins in E. coli host
por: Pouresmaeil, Mahin, et al.
Publicado: (2023)

BFMP: A Method for Discretizing and Visualizing Pyranose Conformations
por: Makeneni, Spandana, et al.
Publicado: (2014)

Quantitative Comparison of Pyranose Dehydrogenase Action on Diverse Xylooligosaccharides
por: Karppi, Johanna, et al.
Publicado: (2020)

The Effects of Lactose Induction on a Plasmid-Free E. coli T7 Expression System
por: Hausjell, Johanna, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_4fe6bcqlorq1lrjk7e0fgj30gt