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Comparison of different methods for preparation and characterization of total RNA from cartilage samples to uncover osteoarthritis in vivo

BACKGROUND: The isolation of intact RNA can be very difficult when tissues are used that contain many RNAses or that are hard to homogenize, e.g. cartilage samples. Additionally, cartilaginous tissues are characterized by a low cellularity and an abundance of extracellular matrix (ECM) molecules. Bu...

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Detalles Bibliográficos
Autores principales: Ruettger, Anke, Neumann, Steffi, Wiederanders, Bernd, Huber, René
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2841606/
https://www.ncbi.nlm.nih.gov/pubmed/20180968
http://dx.doi.org/10.1186/1756-0500-3-7