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Comparison of different methods for preparation and characterization of total RNA from cartilage samples to uncover osteoarthritis in vivo
BACKGROUND: The isolation of intact RNA can be very difficult when tissues are used that contain many RNAses or that are hard to homogenize, e.g. cartilage samples. Additionally, cartilaginous tissues are characterized by a low cellularity and an abundance of extracellular matrix (ECM) molecules. Bu...
Autores principales: | Ruettger, Anke, Neumann, Steffi, Wiederanders, Bernd, Huber, René |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2841606/ https://www.ncbi.nlm.nih.gov/pubmed/20180968 http://dx.doi.org/10.1186/1756-0500-3-7 |
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