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A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry
Viruses use cellular machinery to enter and infect cells. In this study we address the cell entry mechanisms of nonenveloped adenoviruses (Ads). We show that protein VI, an internal capsid protein, is rapidly exposed after cell surface attachment and internalization and remains partially associated...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2841620/ https://www.ncbi.nlm.nih.gov/pubmed/20333243 http://dx.doi.org/10.1371/journal.ppat.1000808 |
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author | Wodrich, Harald Henaff, Daniel Jammart, Baptist Segura-Morales, Carolina Seelmeir, Sigrid Coux, Olivier Ruzsics, Zsolt Wiethoff, Christopher M. Kremer, Eric J. |
author_facet | Wodrich, Harald Henaff, Daniel Jammart, Baptist Segura-Morales, Carolina Seelmeir, Sigrid Coux, Olivier Ruzsics, Zsolt Wiethoff, Christopher M. Kremer, Eric J. |
author_sort | Wodrich, Harald |
collection | PubMed |
description | Viruses use cellular machinery to enter and infect cells. In this study we address the cell entry mechanisms of nonenveloped adenoviruses (Ads). We show that protein VI, an internal capsid protein, is rapidly exposed after cell surface attachment and internalization and remains partially associated with the capsid during intracellular transport. We found that a PPxY motif within protein VI recruits Nedd4 E3 ubiquitin ligases to bind and ubiquitylate protein VI. We further show that this PPxY motif is involved in rapid, microtubule-dependent intracellular movement of protein VI. Ads with a mutated PPxY motif can efficiently escape endosomes but are defective in microtubule-dependent trafficking toward the nucleus. Likewise, depletion of Nedd4 ligases attenuates nuclear accumulation of incoming Ad particles and infection. Our data provide the first evidence that virus-encoded PPxY motifs are required during virus entry, which may be of significance for several other pathogens. |
format | Text |
id | pubmed-2841620 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28416202010-03-24 A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry Wodrich, Harald Henaff, Daniel Jammart, Baptist Segura-Morales, Carolina Seelmeir, Sigrid Coux, Olivier Ruzsics, Zsolt Wiethoff, Christopher M. Kremer, Eric J. PLoS Pathog Research Article Viruses use cellular machinery to enter and infect cells. In this study we address the cell entry mechanisms of nonenveloped adenoviruses (Ads). We show that protein VI, an internal capsid protein, is rapidly exposed after cell surface attachment and internalization and remains partially associated with the capsid during intracellular transport. We found that a PPxY motif within protein VI recruits Nedd4 E3 ubiquitin ligases to bind and ubiquitylate protein VI. We further show that this PPxY motif is involved in rapid, microtubule-dependent intracellular movement of protein VI. Ads with a mutated PPxY motif can efficiently escape endosomes but are defective in microtubule-dependent trafficking toward the nucleus. Likewise, depletion of Nedd4 ligases attenuates nuclear accumulation of incoming Ad particles and infection. Our data provide the first evidence that virus-encoded PPxY motifs are required during virus entry, which may be of significance for several other pathogens. Public Library of Science 2010-03-19 /pmc/articles/PMC2841620/ /pubmed/20333243 http://dx.doi.org/10.1371/journal.ppat.1000808 Text en Wodrich et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wodrich, Harald Henaff, Daniel Jammart, Baptist Segura-Morales, Carolina Seelmeir, Sigrid Coux, Olivier Ruzsics, Zsolt Wiethoff, Christopher M. Kremer, Eric J. A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry |
title | A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry |
title_full | A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry |
title_fullStr | A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry |
title_full_unstemmed | A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry |
title_short | A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry |
title_sort | capsid-encoded ppxy-motif facilitates adenovirus entry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2841620/ https://www.ncbi.nlm.nih.gov/pubmed/20333243 http://dx.doi.org/10.1371/journal.ppat.1000808 |
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