A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation

We describe a novel method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the C. jejuni glycosylation machinery in E. coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed a...

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Detalles Bibliográficos
Autores principales: Schwarz, Flavio, Huang, Wei, Li, Cishan, Schulz, Benjamin L., Lizak, Christian, Palumbo, Alessandro, Numao, Shin, Neri, Dario, Aebi, Markus, Wang, Lai-Xi
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2842479/
https://www.ncbi.nlm.nih.gov/pubmed/20190762
http://dx.doi.org/10.1038/nchembio.314
Descripción
Sumario:We describe a novel method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the C. jejuni glycosylation machinery in E. coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. It provides a potentially general platform for producing eukaryotic N-glycoproteins.

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