A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation

We describe a novel method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the C. jejuni glycosylation machinery in E. coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed a...

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Detalles Bibliográficos
Autores principales: Schwarz, Flavio, Huang, Wei, Li, Cishan, Schulz, Benjamin L., Lizak, Christian, Palumbo, Alessandro, Numao, Shin, Neri, Dario, Aebi, Markus, Wang, Lai-Xi
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2842479/
https://www.ncbi.nlm.nih.gov/pubmed/20190762
http://dx.doi.org/10.1038/nchembio.314
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author Schwarz, Flavio
Huang, Wei
Li, Cishan
Schulz, Benjamin L.
Lizak, Christian
Palumbo, Alessandro
Numao, Shin
Neri, Dario
Aebi, Markus
Wang, Lai-Xi
author_facet Schwarz, Flavio
Huang, Wei
Li, Cishan
Schulz, Benjamin L.
Lizak, Christian
Palumbo, Alessandro
Numao, Shin
Neri, Dario
Aebi, Markus
Wang, Lai-Xi
author_sort Schwarz, Flavio
collection PubMed
description We describe a novel method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the C. jejuni glycosylation machinery in E. coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. It provides a potentially general platform for producing eukaryotic N-glycoproteins.
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spelling pubmed-28424792010-10-01 A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation Schwarz, Flavio Huang, Wei Li, Cishan Schulz, Benjamin L. Lizak, Christian Palumbo, Alessandro Numao, Shin Neri, Dario Aebi, Markus Wang, Lai-Xi Nat Chem Biol Article We describe a novel method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the C. jejuni glycosylation machinery in E. coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. It provides a potentially general platform for producing eukaryotic N-glycoproteins. 2010-02-28 2010-04 /pmc/articles/PMC2842479/ /pubmed/20190762 http://dx.doi.org/10.1038/nchembio.314 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Schwarz, Flavio
Huang, Wei
Li, Cishan
Schulz, Benjamin L.
Lizak, Christian
Palumbo, Alessandro
Numao, Shin
Neri, Dario
Aebi, Markus
Wang, Lai-Xi
A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation
title A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation
title_full A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation
title_fullStr A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation
title_full_unstemmed A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation
title_short A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation
title_sort combined method for producing homogeneous glycoproteins with eukaryotic n-glycosylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2842479/
https://www.ncbi.nlm.nih.gov/pubmed/20190762
http://dx.doi.org/10.1038/nchembio.314
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