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A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation
We describe a novel method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the C. jejuni glycosylation machinery in E. coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed a...
| Autores principales: | , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2842479/ https://www.ncbi.nlm.nih.gov/pubmed/20190762 http://dx.doi.org/10.1038/nchembio.314 |