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Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection

BACKGROUND: Porcine proliferative enteropathy in pigs is caused by the obligate, intracellular bacterium Lawsonia intracellularis. In vitro studies have shown close bacterium-cell interaction followed by cellular uptake of the bacterium within 3 h post inoculation (PI). However, knowledge of the ini...

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Autores principales: Boutrup, Torsten S, Schauser, Kirsten, Agerholm, Jørgen S, Jensen, Tim K
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2843685/
https://www.ncbi.nlm.nih.gov/pubmed/20181246
http://dx.doi.org/10.1186/1751-0147-52-17
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author Boutrup, Torsten S
Schauser, Kirsten
Agerholm, Jørgen S
Jensen, Tim K
author_facet Boutrup, Torsten S
Schauser, Kirsten
Agerholm, Jørgen S
Jensen, Tim K
author_sort Boutrup, Torsten S
collection PubMed
description BACKGROUND: Porcine proliferative enteropathy in pigs is caused by the obligate, intracellular bacterium Lawsonia intracellularis. In vitro studies have shown close bacterium-cell interaction followed by cellular uptake of the bacterium within 3 h post inoculation (PI). However, knowledge of the initial in vivo interaction between porcine intestinal epithelium and the bacterium is limited. The aims of the present study were to evaluate the usefulness of a ligated small intestinal loop model to study L. intracellularis infections and to obtain information on the very early L. intracellularis-enterocyte interactions. METHODS: A ligated small intestinal loop model using three different L. intracellularis inocula was applied to 10-11-week-old pigs. The inocula were 1) wild type bacteria derived from overnight incubation of L. intracellularis bacteria from spontaneous disease, 2) crude vaccine bacteria (Enterisol(® )Ileitis Vet), and 3) vaccine bacteria propagated in cell culture. The bacteria-enterocyte interaction was visualised using immunohistochemistry on specimens derived 1, 3 and 6 h PI respectively. RESULTS: Although at a low level, close contact between bacteria and the enterocyte brush border including intracellular uptake of bacteria in mature enterocytes was seen at 3 and 6 h PI for the vaccine and the propagated vaccine inocula. Interaction between the wild-type bacteria and villus enterocytes was scarce and only seen at 6 h PI, where a few bacteria were found in close contact with the brush border. CONCLUSIONS: The ligated intestinal loop model was useful with respect to maintaining an intact intestinal morphology for up to 6 h. Furthermore, the study demonstrated that L. intracellularis interacts with villus enterocytes within 3 to 6 h after inoculation into intestinal loops and that the bacterium, as shown for the vaccine bacteria, propagated as well as non-propagated, was able to invade mature enterocytes. Thus, the study demonstrates the early intestinal invasion of L. intracellularis in vivo.
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spelling pubmed-28436852010-03-23 Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection Boutrup, Torsten S Schauser, Kirsten Agerholm, Jørgen S Jensen, Tim K Acta Vet Scand Research BACKGROUND: Porcine proliferative enteropathy in pigs is caused by the obligate, intracellular bacterium Lawsonia intracellularis. In vitro studies have shown close bacterium-cell interaction followed by cellular uptake of the bacterium within 3 h post inoculation (PI). However, knowledge of the initial in vivo interaction between porcine intestinal epithelium and the bacterium is limited. The aims of the present study were to evaluate the usefulness of a ligated small intestinal loop model to study L. intracellularis infections and to obtain information on the very early L. intracellularis-enterocyte interactions. METHODS: A ligated small intestinal loop model using three different L. intracellularis inocula was applied to 10-11-week-old pigs. The inocula were 1) wild type bacteria derived from overnight incubation of L. intracellularis bacteria from spontaneous disease, 2) crude vaccine bacteria (Enterisol(® )Ileitis Vet), and 3) vaccine bacteria propagated in cell culture. The bacteria-enterocyte interaction was visualised using immunohistochemistry on specimens derived 1, 3 and 6 h PI respectively. RESULTS: Although at a low level, close contact between bacteria and the enterocyte brush border including intracellular uptake of bacteria in mature enterocytes was seen at 3 and 6 h PI for the vaccine and the propagated vaccine inocula. Interaction between the wild-type bacteria and villus enterocytes was scarce and only seen at 6 h PI, where a few bacteria were found in close contact with the brush border. CONCLUSIONS: The ligated intestinal loop model was useful with respect to maintaining an intact intestinal morphology for up to 6 h. Furthermore, the study demonstrated that L. intracellularis interacts with villus enterocytes within 3 to 6 h after inoculation into intestinal loops and that the bacterium, as shown for the vaccine bacteria, propagated as well as non-propagated, was able to invade mature enterocytes. Thus, the study demonstrates the early intestinal invasion of L. intracellularis in vivo. BioMed Central 2010-02-24 /pmc/articles/PMC2843685/ /pubmed/20181246 http://dx.doi.org/10.1186/1751-0147-52-17 Text en Copyright ©2010 Boutrup et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Boutrup, Torsten S
Schauser, Kirsten
Agerholm, Jørgen S
Jensen, Tim K
Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection
title Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection
title_full Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection
title_fullStr Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection
title_full_unstemmed Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection
title_short Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection
title_sort application of a pig ligated intestinal loop model for early lawsonia intracellularis infection
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2843685/
https://www.ncbi.nlm.nih.gov/pubmed/20181246
http://dx.doi.org/10.1186/1751-0147-52-17
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