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Characterization of the retinal proteome during rod photoreceptor genesis

BACKGROUND: The process of rod photoreceptor genesis, cell fate determination and differentiation is complex and multi-factorial. Previous studies have defined a model of photoreceptor differentiation that relies on intrinsic changes within the presumptive photoreceptor cells as well as changes in s...

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Autores principales: Barnhill, Alison E, Hecker, Laura A, Kohutyuk, Oksana, Buss, Janice E, Honavar, Vasant G, Greenlee, Heather West
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2843734/
https://www.ncbi.nlm.nih.gov/pubmed/20181029
http://dx.doi.org/10.1186/1756-0500-3-25
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author Barnhill, Alison E
Hecker, Laura A
Kohutyuk, Oksana
Buss, Janice E
Honavar, Vasant G
Greenlee, Heather West
author_facet Barnhill, Alison E
Hecker, Laura A
Kohutyuk, Oksana
Buss, Janice E
Honavar, Vasant G
Greenlee, Heather West
author_sort Barnhill, Alison E
collection PubMed
description BACKGROUND: The process of rod photoreceptor genesis, cell fate determination and differentiation is complex and multi-factorial. Previous studies have defined a model of photoreceptor differentiation that relies on intrinsic changes within the presumptive photoreceptor cells as well as changes in surrounding tissue that are extrinsic to the cell. We have used a proteomics approach to identify proteins that are dynamically expressed in the mouse retina during rod genesis and differentiation. FINDINGS: A series of six developmental ages from E13 to P5 were used to define changes in retinal protein expression during rod photoreceptor genesis and early differentiation. Retinal proteins were separated by isoelectric focus point and molecular weight. Gels were analyzed for changes in protein spot intensity across developmental time. Protein spots that peaked in expression at E17, P0 and P5 were picked from gels for identification. There were 239 spots that were picked for identification based on their dynamic expression during the developmental period of maximal rod photoreceptor genesis and differentiation. Of the 239 spots, 60 of them were reliably identified and represented a single protein. Ten proteins were represented by multiple spots, suggesting they were post-translationally modified. Of the 42 unique dynamically expressed proteins identified, 16 had been previously reported to be associated with the developing retina. CONCLUSIONS: Our results represent the first proteomics study of the developing mouse retina that includes prenatal development. We identified 26 dynamically expressed proteins in the developing mouse retina whose expression had not been previously associated with retinal development.
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spelling pubmed-28437342010-03-23 Characterization of the retinal proteome during rod photoreceptor genesis Barnhill, Alison E Hecker, Laura A Kohutyuk, Oksana Buss, Janice E Honavar, Vasant G Greenlee, Heather West BMC Res Notes Short Report BACKGROUND: The process of rod photoreceptor genesis, cell fate determination and differentiation is complex and multi-factorial. Previous studies have defined a model of photoreceptor differentiation that relies on intrinsic changes within the presumptive photoreceptor cells as well as changes in surrounding tissue that are extrinsic to the cell. We have used a proteomics approach to identify proteins that are dynamically expressed in the mouse retina during rod genesis and differentiation. FINDINGS: A series of six developmental ages from E13 to P5 were used to define changes in retinal protein expression during rod photoreceptor genesis and early differentiation. Retinal proteins were separated by isoelectric focus point and molecular weight. Gels were analyzed for changes in protein spot intensity across developmental time. Protein spots that peaked in expression at E17, P0 and P5 were picked from gels for identification. There were 239 spots that were picked for identification based on their dynamic expression during the developmental period of maximal rod photoreceptor genesis and differentiation. Of the 239 spots, 60 of them were reliably identified and represented a single protein. Ten proteins were represented by multiple spots, suggesting they were post-translationally modified. Of the 42 unique dynamically expressed proteins identified, 16 had been previously reported to be associated with the developing retina. CONCLUSIONS: Our results represent the first proteomics study of the developing mouse retina that includes prenatal development. We identified 26 dynamically expressed proteins in the developing mouse retina whose expression had not been previously associated with retinal development. BioMed Central 2010-01-27 /pmc/articles/PMC2843734/ /pubmed/20181029 http://dx.doi.org/10.1186/1756-0500-3-25 Text en Copyright ©2010 Greenlee et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Barnhill, Alison E
Hecker, Laura A
Kohutyuk, Oksana
Buss, Janice E
Honavar, Vasant G
Greenlee, Heather West
Characterization of the retinal proteome during rod photoreceptor genesis
title Characterization of the retinal proteome during rod photoreceptor genesis
title_full Characterization of the retinal proteome during rod photoreceptor genesis
title_fullStr Characterization of the retinal proteome during rod photoreceptor genesis
title_full_unstemmed Characterization of the retinal proteome during rod photoreceptor genesis
title_short Characterization of the retinal proteome during rod photoreceptor genesis
title_sort characterization of the retinal proteome during rod photoreceptor genesis
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2843734/
https://www.ncbi.nlm.nih.gov/pubmed/20181029
http://dx.doi.org/10.1186/1756-0500-3-25
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