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PCS-based structure determination of protein–protein complexes

A simple and fast nuclear magnetic resonance method for docking proteins using pseudo-contact shift (PCS) and (1)H(N)/(15)N chemical shift perturbation is presented. PCS is induced by a paramagnetic lanthanide ion that is attached to a target protein using a lanthanide binding peptide tag anchored a...

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Detalles Bibliográficos
Autores principales: Saio, Tomohide, Yokochi, Masashi, Kumeta, Hiroyuki, Inagaki, Fuyuhiko
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2844537/
https://www.ncbi.nlm.nih.gov/pubmed/20300805
http://dx.doi.org/10.1007/s10858-010-9401-4

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