Detecting the conformation of individual proteins in live cells

We combined single molecule fluorescence resonance energy transfer (smFRET) with single particle tracking in live cells to detect the in vivo conformation of individual proteins. We site-specifically labeled recombinant SNARE proteins with a FRET donor and acceptor before microinjecting them into cu...

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Detalles Bibliográficos
Autores principales: Sakon, John J., Weninger, Keith R.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2844853/
https://www.ncbi.nlm.nih.gov/pubmed/20118931
http://dx.doi.org/10.1038/nmeth.1421
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author Sakon, John J.
Weninger, Keith R.
author_facet Sakon, John J.
Weninger, Keith R.
author_sort Sakon, John J.
collection PubMed
description We combined single molecule fluorescence resonance energy transfer (smFRET) with single particle tracking in live cells to detect the in vivo conformation of individual proteins. We site-specifically labeled recombinant SNARE proteins with a FRET donor and acceptor before microinjecting them into cultured cells. We observed that individual proteins rapidly incorporated into folded complexes at the cell membrane, demonstrating the potential of this method to reveal dynamic interactions within cells.
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spelling pubmed-28448532010-09-01 Detecting the conformation of individual proteins in live cells Sakon, John J. Weninger, Keith R. Nat Methods Article We combined single molecule fluorescence resonance energy transfer (smFRET) with single particle tracking in live cells to detect the in vivo conformation of individual proteins. We site-specifically labeled recombinant SNARE proteins with a FRET donor and acceptor before microinjecting them into cultured cells. We observed that individual proteins rapidly incorporated into folded complexes at the cell membrane, demonstrating the potential of this method to reveal dynamic interactions within cells. 2010-01-31 2010-03 /pmc/articles/PMC2844853/ /pubmed/20118931 http://dx.doi.org/10.1038/nmeth.1421 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Sakon, John J.
Weninger, Keith R.
Detecting the conformation of individual proteins in live cells
title Detecting the conformation of individual proteins in live cells
title_full Detecting the conformation of individual proteins in live cells
title_fullStr Detecting the conformation of individual proteins in live cells
title_full_unstemmed Detecting the conformation of individual proteins in live cells
title_short Detecting the conformation of individual proteins in live cells
title_sort detecting the conformation of individual proteins in live cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2844853/
https://www.ncbi.nlm.nih.gov/pubmed/20118931
http://dx.doi.org/10.1038/nmeth.1421
work_keys_str_mv AT sakonjohnj detectingtheconformationofindividualproteinsinlivecells
AT weningerkeithr detectingtheconformationofindividualproteinsinlivecells

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