Heterologous expression of leader-less pga gene in Pichia pastoris: intracellular production of prokaryotic enzyme

BACKGROUND: Penicillin G acylase of Escherichia coli (PGA(Ec)) is a commercially valuable enzyme for which efficient bacterial expression systems have been developed. The enzyme is used as a catalyst for the hydrolytic production of β-lactam nuclei or for the synthesis of semi-synthetic penicillins...

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Detalles Bibliográficos
Autores principales: Marešová, Helena, Marková, Zdena, Valešová, Renáta, Sklenář, Jan, Kyslík, Pavel
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845550/
https://www.ncbi.nlm.nih.gov/pubmed/20128906
http://dx.doi.org/10.1186/1472-6750-10-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845550/
https://www.ncbi.nlm.nih.gov/pubmed/20128906
http://dx.doi.org/10.1186/1472-6750-10-7

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