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Heterologous expression of leader-less pga gene in Pichia pastoris: intracellular production of prokaryotic enzyme
BACKGROUND: Penicillin G acylase of Escherichia coli (PGA(Ec)) is a commercially valuable enzyme for which efficient bacterial expression systems have been developed. The enzyme is used as a catalyst for the hydrolytic production of β-lactam nuclei or for the synthesis of semi-synthetic penicillins...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845550/ https://www.ncbi.nlm.nih.gov/pubmed/20128906 http://dx.doi.org/10.1186/1472-6750-10-7 |