Histological Study of Fresh Versus Frozen Semitendinous Muscle Tendon Allografts

OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at −80°C in comparison to a control group kept at only −4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tend...

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Detalles Bibliográficos
Autores principales: Bitar, Alexandre Carneiro, Santos, Luiz Augusto Ubirajara, Croci, Alberto Tesconi, Pereira, João Alberto Ramos Maradei, França Bisneto, Edgard N., Giovani, Arlete Mazzini Miranda, Oliveira, Claudia Regina G. C. M.
Formato: Texto
Lenguaje:English
Publicado: Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2010
Materias:
Basic Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845771/
https://www.ncbi.nlm.nih.gov/pubmed/20360921
http://dx.doi.org/10.1590/S1807-59322010000300010
Descripción
Sumario:OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at −80°C in comparison to a control group kept at only −4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures. METHODS: In a tissue bank, 10 semitendinous tendons from 10 cadavers were frozen at −80ºC as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still “fresh”. RESULTS: There was no histological difference between the fresh and frozen samples in relation to seven variables. CONCLUSIONS: Semitendinous muscle tendon allografts can be submitted to cryopreservation at −80ºC without suffering histological modifications.

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