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Histological Study of Fresh Versus Frozen Semitendinous Muscle Tendon Allografts
OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at −80°C in comparison to a control group kept at only −4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tend...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845771/ https://www.ncbi.nlm.nih.gov/pubmed/20360921 http://dx.doi.org/10.1590/S1807-59322010000300010 |
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author | Bitar, Alexandre Carneiro Santos, Luiz Augusto Ubirajara Croci, Alberto Tesconi Pereira, João Alberto Ramos Maradei França Bisneto, Edgard N. Giovani, Arlete Mazzini Miranda Oliveira, Claudia Regina G. C. M. |
author_facet | Bitar, Alexandre Carneiro Santos, Luiz Augusto Ubirajara Croci, Alberto Tesconi Pereira, João Alberto Ramos Maradei França Bisneto, Edgard N. Giovani, Arlete Mazzini Miranda Oliveira, Claudia Regina G. C. M. |
author_sort | Bitar, Alexandre Carneiro |
collection | PubMed |
description | OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at −80°C in comparison to a control group kept at only −4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures. METHODS: In a tissue bank, 10 semitendinous tendons from 10 cadavers were frozen at −80ºC as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still “fresh”. RESULTS: There was no histological difference between the fresh and frozen samples in relation to seven variables. CONCLUSIONS: Semitendinous muscle tendon allografts can be submitted to cryopreservation at −80ºC without suffering histological modifications. |
format | Text |
id | pubmed-2845771 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo |
record_format | MEDLINE/PubMed |
spelling | pubmed-28457712010-04-01 Histological Study of Fresh Versus Frozen Semitendinous Muscle Tendon Allografts Bitar, Alexandre Carneiro Santos, Luiz Augusto Ubirajara Croci, Alberto Tesconi Pereira, João Alberto Ramos Maradei França Bisneto, Edgard N. Giovani, Arlete Mazzini Miranda Oliveira, Claudia Regina G. C. M. Clinics (Sao Paulo) Basic Research OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at −80°C in comparison to a control group kept at only −4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures. METHODS: In a tissue bank, 10 semitendinous tendons from 10 cadavers were frozen at −80ºC as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still “fresh”. RESULTS: There was no histological difference between the fresh and frozen samples in relation to seven variables. CONCLUSIONS: Semitendinous muscle tendon allografts can be submitted to cryopreservation at −80ºC without suffering histological modifications. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2010-03 /pmc/articles/PMC2845771/ /pubmed/20360921 http://dx.doi.org/10.1590/S1807-59322010000300010 Text en Copyright © 2010 Hospital das Clínicas da FMUSP |
spellingShingle | Basic Research Bitar, Alexandre Carneiro Santos, Luiz Augusto Ubirajara Croci, Alberto Tesconi Pereira, João Alberto Ramos Maradei França Bisneto, Edgard N. Giovani, Arlete Mazzini Miranda Oliveira, Claudia Regina G. C. M. Histological Study of Fresh Versus Frozen Semitendinous Muscle Tendon Allografts |
title | Histological Study of Fresh Versus Frozen Semitendinous Muscle Tendon Allografts |
title_full | Histological Study of Fresh Versus Frozen Semitendinous Muscle Tendon Allografts |
title_fullStr | Histological Study of Fresh Versus Frozen Semitendinous Muscle Tendon Allografts |
title_full_unstemmed | Histological Study of Fresh Versus Frozen Semitendinous Muscle Tendon Allografts |
title_short | Histological Study of Fresh Versus Frozen Semitendinous Muscle Tendon Allografts |
title_sort | histological study of fresh versus frozen semitendinous muscle tendon allografts |
topic | Basic Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845771/ https://www.ncbi.nlm.nih.gov/pubmed/20360921 http://dx.doi.org/10.1590/S1807-59322010000300010 |
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