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Xenograft Transplantation of Human Malignant Astrocytoma Cells Into Immunodeficient Rats: An Experimental Model of Glioblastoma

INTRODUCTION: Astrocytic gliomas are the most common intracranial central nervous system neoplasias, accounting for about 60% of all primary central nervous system tumors. Despite advances in the treatment of gliomas, no effective therapeutic approach is yet available; hence, the search for a more r...

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Autores principales: Miura, Flávio Key, Alves, Maria Jose Ferreira, Rocha, Mussya Cisotto, da Silva, Roseli, Oba-Shinjo, Sueli Mieko, Marie, Suely Kazue Nagahashi
Formato: Texto
Lenguaje:English
Publicado: Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845772/
https://www.ncbi.nlm.nih.gov/pubmed/20360922
http://dx.doi.org/10.1590/S1807-59322010000300011
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author Miura, Flávio Key
Alves, Maria Jose Ferreira
Rocha, Mussya Cisotto
da Silva, Roseli
Oba-Shinjo, Sueli Mieko
Marie, Suely Kazue Nagahashi
author_facet Miura, Flávio Key
Alves, Maria Jose Ferreira
Rocha, Mussya Cisotto
da Silva, Roseli
Oba-Shinjo, Sueli Mieko
Marie, Suely Kazue Nagahashi
author_sort Miura, Flávio Key
collection PubMed
description INTRODUCTION: Astrocytic gliomas are the most common intracranial central nervous system neoplasias, accounting for about 60% of all primary central nervous system tumors. Despite advances in the treatment of gliomas, no effective therapeutic approach is yet available; hence, the search for a more realistic model to generate more effective therapies is essential. OBJECTIVE: To develop an experimental malignant astrocytoma model with the characteristics of the human tumor. METHOD: Primary cells from subcutaneous xenograft tumors produced with malignant astrocytoma U87MG cells were inoculated intracerebrally by stereotaxis into immunosuppressed (athymic) Rowett rats. RESULTS: All four injected animals developed non-infiltrative tumors, although other glioblastoma characteristics, such as necrosis, pseudopalisading cells and intense mitotic activity, were observed. CONCLUSION: A malignant astrocytoma intracerebral xenograft model with poorly invasive behavior was achieved in athymic Rowett rats. Tumor invasiveness in an experimental animal model may depend on a combination of several factors, including the cell line used to induce tumor formation, the rat strains and the status of the animal’s immune system.
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spelling pubmed-28457722010-04-01 Xenograft Transplantation of Human Malignant Astrocytoma Cells Into Immunodeficient Rats: An Experimental Model of Glioblastoma Miura, Flávio Key Alves, Maria Jose Ferreira Rocha, Mussya Cisotto da Silva, Roseli Oba-Shinjo, Sueli Mieko Marie, Suely Kazue Nagahashi Clinics (Sao Paulo) Basic Research INTRODUCTION: Astrocytic gliomas are the most common intracranial central nervous system neoplasias, accounting for about 60% of all primary central nervous system tumors. Despite advances in the treatment of gliomas, no effective therapeutic approach is yet available; hence, the search for a more realistic model to generate more effective therapies is essential. OBJECTIVE: To develop an experimental malignant astrocytoma model with the characteristics of the human tumor. METHOD: Primary cells from subcutaneous xenograft tumors produced with malignant astrocytoma U87MG cells were inoculated intracerebrally by stereotaxis into immunosuppressed (athymic) Rowett rats. RESULTS: All four injected animals developed non-infiltrative tumors, although other glioblastoma characteristics, such as necrosis, pseudopalisading cells and intense mitotic activity, were observed. CONCLUSION: A malignant astrocytoma intracerebral xenograft model with poorly invasive behavior was achieved in athymic Rowett rats. Tumor invasiveness in an experimental animal model may depend on a combination of several factors, including the cell line used to induce tumor formation, the rat strains and the status of the animal’s immune system. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2010-03 /pmc/articles/PMC2845772/ /pubmed/20360922 http://dx.doi.org/10.1590/S1807-59322010000300011 Text en Copyright © 2010 Hospital das Clínicas da FMUSP
spellingShingle Basic Research
Miura, Flávio Key
Alves, Maria Jose Ferreira
Rocha, Mussya Cisotto
da Silva, Roseli
Oba-Shinjo, Sueli Mieko
Marie, Suely Kazue Nagahashi
Xenograft Transplantation of Human Malignant Astrocytoma Cells Into Immunodeficient Rats: An Experimental Model of Glioblastoma
title Xenograft Transplantation of Human Malignant Astrocytoma Cells Into Immunodeficient Rats: An Experimental Model of Glioblastoma
title_full Xenograft Transplantation of Human Malignant Astrocytoma Cells Into Immunodeficient Rats: An Experimental Model of Glioblastoma
title_fullStr Xenograft Transplantation of Human Malignant Astrocytoma Cells Into Immunodeficient Rats: An Experimental Model of Glioblastoma
title_full_unstemmed Xenograft Transplantation of Human Malignant Astrocytoma Cells Into Immunodeficient Rats: An Experimental Model of Glioblastoma
title_short Xenograft Transplantation of Human Malignant Astrocytoma Cells Into Immunodeficient Rats: An Experimental Model of Glioblastoma
title_sort xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845772/
https://www.ncbi.nlm.nih.gov/pubmed/20360922
http://dx.doi.org/10.1590/S1807-59322010000300011
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