Cargando…
Functional genomics of hsp-90 in parasitic and free-living nematodes
Heat shock protein 90 (Hsp-90) is a highly conserved essential protein in eukaryotes. Here we describe the molecular characterisation of hsp-90 from three nematodes, the free-living Caenorhabditis elegans (Ce) and the parasitic worms Brugia pahangi (Bp) and Haemonchus contortus (Hc). These molecules...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845815/ https://www.ncbi.nlm.nih.gov/pubmed/19401205 http://dx.doi.org/10.1016/j.ijpara.2009.02.024 |
_version_ | 1782179444068188160 |
---|---|
author | Gillan, Victoria Maitland, Kirsty McCormack, Gillian Nik Him, Nik A.I.I. Devaney, Eileen |
author_facet | Gillan, Victoria Maitland, Kirsty McCormack, Gillian Nik Him, Nik A.I.I. Devaney, Eileen |
author_sort | Gillan, Victoria |
collection | PubMed |
description | Heat shock protein 90 (Hsp-90) is a highly conserved essential protein in eukaryotes. Here we describe the molecular characterisation of hsp-90 from three nematodes, the free-living Caenorhabditis elegans (Ce) and the parasitic worms Brugia pahangi (Bp) and Haemonchus contortus (Hc). These molecules were functionally characterised by rescue of a Ce-daf-21 (hsp-90) null mutant. Our results show a gradient of rescue: the C. elegans endogenous gene provided full rescue of the daf-21 mutant, while Hc-hsp-90 provided partial rescue. In contrast, no rescue could be obtained using a variety of Bp-hsp-90 constructs, despite the fact that Bp-hsp-90 was transcribed and translated in the mutant worms. daf-21 RNA interference (RNAi) experiments were carried out to determine whether knock-down of the endogenous daf-21 mRNA in N2 worms could be complemented by expression of either parasite gene. However neither parasite gene could rescue the daf-21 (RNAi) phenotypes. These results indicate that factors other than the level of sequence identity are important for determining whether parasite genes can functionally complement in C. elegans. |
format | Text |
id | pubmed-2845815 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Elsevier Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28458152010-03-31 Functional genomics of hsp-90 in parasitic and free-living nematodes Gillan, Victoria Maitland, Kirsty McCormack, Gillian Nik Him, Nik A.I.I. Devaney, Eileen Int J Parasitol Article Heat shock protein 90 (Hsp-90) is a highly conserved essential protein in eukaryotes. Here we describe the molecular characterisation of hsp-90 from three nematodes, the free-living Caenorhabditis elegans (Ce) and the parasitic worms Brugia pahangi (Bp) and Haemonchus contortus (Hc). These molecules were functionally characterised by rescue of a Ce-daf-21 (hsp-90) null mutant. Our results show a gradient of rescue: the C. elegans endogenous gene provided full rescue of the daf-21 mutant, while Hc-hsp-90 provided partial rescue. In contrast, no rescue could be obtained using a variety of Bp-hsp-90 constructs, despite the fact that Bp-hsp-90 was transcribed and translated in the mutant worms. daf-21 RNA interference (RNAi) experiments were carried out to determine whether knock-down of the endogenous daf-21 mRNA in N2 worms could be complemented by expression of either parasite gene. However neither parasite gene could rescue the daf-21 (RNAi) phenotypes. These results indicate that factors other than the level of sequence identity are important for determining whether parasite genes can functionally complement in C. elegans. Elsevier Science 2009-08 /pmc/articles/PMC2845815/ /pubmed/19401205 http://dx.doi.org/10.1016/j.ijpara.2009.02.024 Text en © 2009 Elsevier Ltd. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Article Gillan, Victoria Maitland, Kirsty McCormack, Gillian Nik Him, Nik A.I.I. Devaney, Eileen Functional genomics of hsp-90 in parasitic and free-living nematodes |
title | Functional genomics of hsp-90 in parasitic and free-living nematodes |
title_full | Functional genomics of hsp-90 in parasitic and free-living nematodes |
title_fullStr | Functional genomics of hsp-90 in parasitic and free-living nematodes |
title_full_unstemmed | Functional genomics of hsp-90 in parasitic and free-living nematodes |
title_short | Functional genomics of hsp-90 in parasitic and free-living nematodes |
title_sort | functional genomics of hsp-90 in parasitic and free-living nematodes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845815/ https://www.ncbi.nlm.nih.gov/pubmed/19401205 http://dx.doi.org/10.1016/j.ijpara.2009.02.024 |
work_keys_str_mv | AT gillanvictoria functionalgenomicsofhsp90inparasiticandfreelivingnematodes AT maitlandkirsty functionalgenomicsofhsp90inparasiticandfreelivingnematodes AT mccormackgillian functionalgenomicsofhsp90inparasiticandfreelivingnematodes AT nikhimnikaii functionalgenomicsofhsp90inparasiticandfreelivingnematodes AT devaneyeileen functionalgenomicsofhsp90inparasiticandfreelivingnematodes |