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Protein tyrosine phosphatases expression during development of mouse superior colliculus

Protein tyrosine phosphatases (PTPs) are key regulators of different processes during development of the central nervous system. However, expression patterns and potential roles of PTPs in the developing superior colliculus remain poorly investigated. In this study, a degenerate primer-based reverse...

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Autores principales: Reinhard, Jacqueline, Horvat-Bröcker, Andrea, Illes, Sebastian, Zaremba, Angelika, Knyazev, Piotr, Ullrich, Axel, Faissner, Andreas
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845883/
https://www.ncbi.nlm.nih.gov/pubmed/19727691
http://dx.doi.org/10.1007/s00221-009-1963-6
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author Reinhard, Jacqueline
Horvat-Bröcker, Andrea
Illes, Sebastian
Zaremba, Angelika
Knyazev, Piotr
Ullrich, Axel
Faissner, Andreas
author_facet Reinhard, Jacqueline
Horvat-Bröcker, Andrea
Illes, Sebastian
Zaremba, Angelika
Knyazev, Piotr
Ullrich, Axel
Faissner, Andreas
author_sort Reinhard, Jacqueline
collection PubMed
description Protein tyrosine phosphatases (PTPs) are key regulators of different processes during development of the central nervous system. However, expression patterns and potential roles of PTPs in the developing superior colliculus remain poorly investigated. In this study, a degenerate primer-based reverse transcription-polymerase chain reaction (RT-PCR) approach was used to isolate seven different intracellular PTPs and nine different receptor-type PTPs (RPTPs) from embryonic E15 mouse superior colliculus. Subsequently, the expression patterns of 11 PTPs (TC-PTP, PTP1C, PTP1D, PTP-MEG2, PTP-PEST, RPTPJ, RPTPε, RPTPRR, RPTPσ, RPTPκ and RPTPγ) were further analyzed in detail in superior colliculus from embryonic E13 to postnatal P20 stages by quantitative real-time RT-PCR, Western blotting and immunohistochemistry. Each of the 11 PTPs exhibits distinct spatiotemporal regulation of mRNAs and proteins in the developing superior colliculus suggesting their versatile roles in genesis of neuronal and glial cells and retinocollicular topographic mapping. At E13, additional double-immunohistochemical analysis revealed the expression of PTPs in collicular nestin-positive neural progenitor cells and RC-2-immunoreactive radial glia cells, indicating the potential functional importance of PTPs in neurogenesis and gliogenesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00221-009-1963-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-28458832010-04-05 Protein tyrosine phosphatases expression during development of mouse superior colliculus Reinhard, Jacqueline Horvat-Bröcker, Andrea Illes, Sebastian Zaremba, Angelika Knyazev, Piotr Ullrich, Axel Faissner, Andreas Exp Brain Res Research Article Protein tyrosine phosphatases (PTPs) are key regulators of different processes during development of the central nervous system. However, expression patterns and potential roles of PTPs in the developing superior colliculus remain poorly investigated. In this study, a degenerate primer-based reverse transcription-polymerase chain reaction (RT-PCR) approach was used to isolate seven different intracellular PTPs and nine different receptor-type PTPs (RPTPs) from embryonic E15 mouse superior colliculus. Subsequently, the expression patterns of 11 PTPs (TC-PTP, PTP1C, PTP1D, PTP-MEG2, PTP-PEST, RPTPJ, RPTPε, RPTPRR, RPTPσ, RPTPκ and RPTPγ) were further analyzed in detail in superior colliculus from embryonic E13 to postnatal P20 stages by quantitative real-time RT-PCR, Western blotting and immunohistochemistry. Each of the 11 PTPs exhibits distinct spatiotemporal regulation of mRNAs and proteins in the developing superior colliculus suggesting their versatile roles in genesis of neuronal and glial cells and retinocollicular topographic mapping. At E13, additional double-immunohistochemical analysis revealed the expression of PTPs in collicular nestin-positive neural progenitor cells and RC-2-immunoreactive radial glia cells, indicating the potential functional importance of PTPs in neurogenesis and gliogenesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00221-009-1963-6) contains supplementary material, which is available to authorized users. Springer-Verlag 2009-09-01 2009-12 /pmc/articles/PMC2845883/ /pubmed/19727691 http://dx.doi.org/10.1007/s00221-009-1963-6 Text en © Springer-Verlag 2009
spellingShingle Research Article
Reinhard, Jacqueline
Horvat-Bröcker, Andrea
Illes, Sebastian
Zaremba, Angelika
Knyazev, Piotr
Ullrich, Axel
Faissner, Andreas
Protein tyrosine phosphatases expression during development of mouse superior colliculus
title Protein tyrosine phosphatases expression during development of mouse superior colliculus
title_full Protein tyrosine phosphatases expression during development of mouse superior colliculus
title_fullStr Protein tyrosine phosphatases expression during development of mouse superior colliculus
title_full_unstemmed Protein tyrosine phosphatases expression during development of mouse superior colliculus
title_short Protein tyrosine phosphatases expression during development of mouse superior colliculus
title_sort protein tyrosine phosphatases expression during development of mouse superior colliculus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845883/
https://www.ncbi.nlm.nih.gov/pubmed/19727691
http://dx.doi.org/10.1007/s00221-009-1963-6
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