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Genetic variation in the adiponectin receptor 2 (ADIPOR2) gene is associated with coronary artery disease and increased ADIPOR2 expression in peripheral monocytes
BACKGROUND: Adiponectin is an adipose tissue secreted protein known for its insulin sensitising and anti-atherogenic actions. To this date two adiponectin receptors have been discovered, adiponectin receptor 1 (ADIPOR1) and adiponectin receptor 2 (ADIPOR2). The aim of this study was to investigate t...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2846898/ https://www.ncbi.nlm.nih.gov/pubmed/20178558 http://dx.doi.org/10.1186/1475-2840-9-10 |
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author | Halvatsiotis, Iosif Tsiotra, Panayoula C Ikonomidis, Ignatios Kollias, Anastasios Mitrou, Panagiota Maratou, Eirini Boutati, Eleni Lekakis, John Dimitriadis, George Economopoulos, Theofanis Kremastinos, Dimitrios T Raptis, Sotirios A |
author_facet | Halvatsiotis, Iosif Tsiotra, Panayoula C Ikonomidis, Ignatios Kollias, Anastasios Mitrou, Panagiota Maratou, Eirini Boutati, Eleni Lekakis, John Dimitriadis, George Economopoulos, Theofanis Kremastinos, Dimitrios T Raptis, Sotirios A |
author_sort | Halvatsiotis, Iosif |
collection | PubMed |
description | BACKGROUND: Adiponectin is an adipose tissue secreted protein known for its insulin sensitising and anti-atherogenic actions. To this date two adiponectin receptors have been discovered, adiponectin receptor 1 (ADIPOR1) and adiponectin receptor 2 (ADIPOR2). The aim of this study was to investigate the association of ADIPOR2 gene variations with coronary artery disease (CAD). METHODS: Eight common single nucleotide polymorphisms (SNPs) spanning the entire ADIPOR2 locus were chosen to perform association studies with anthropometric and metabolic parameters in a Greek population. They were classified as either CAD (stenosis >50% in at least one main vessel) or non-CAD individuals in accordance with coronary angiography data. Genotyping was performed using a microsphere-based suspension array and the Allele Specific Primer Extension (ASPE) method. Expression of ADIPOR2 protein and mRNA in circulating CD14(+ )monocytes were determined using flow cytometry and real time Polymerase Chain Reaction assays respectively. RESULTS: There was a significant difference in the distribution of genotypes of polymorphism rs767870 of ADIPOR2 between CAD and non-CAD individuals (p = 0.017). Furthermore, heterozygotes of the rs767870 polymorphism had significantly lower Flow Mediated Dilatation (FMD) values, higher values of Intima-Media Thickness (IMT) and increased ADIPOR2 protein levels in peripheral monocytes, compared to homozygotes of the minor allele after adjustment for age, sex, waist to hip ratio and HOMA. CONCLUSIONS: Our findings suggest that variants of ADIPOR2 could be a determinant for atherosclerosis independent of insulin resistance status, possibly by affecting ADIPOR2 protein levels. |
format | Text |
id | pubmed-2846898 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28468982010-03-30 Genetic variation in the adiponectin receptor 2 (ADIPOR2) gene is associated with coronary artery disease and increased ADIPOR2 expression in peripheral monocytes Halvatsiotis, Iosif Tsiotra, Panayoula C Ikonomidis, Ignatios Kollias, Anastasios Mitrou, Panagiota Maratou, Eirini Boutati, Eleni Lekakis, John Dimitriadis, George Economopoulos, Theofanis Kremastinos, Dimitrios T Raptis, Sotirios A Cardiovasc Diabetol Original investigation BACKGROUND: Adiponectin is an adipose tissue secreted protein known for its insulin sensitising and anti-atherogenic actions. To this date two adiponectin receptors have been discovered, adiponectin receptor 1 (ADIPOR1) and adiponectin receptor 2 (ADIPOR2). The aim of this study was to investigate the association of ADIPOR2 gene variations with coronary artery disease (CAD). METHODS: Eight common single nucleotide polymorphisms (SNPs) spanning the entire ADIPOR2 locus were chosen to perform association studies with anthropometric and metabolic parameters in a Greek population. They were classified as either CAD (stenosis >50% in at least one main vessel) or non-CAD individuals in accordance with coronary angiography data. Genotyping was performed using a microsphere-based suspension array and the Allele Specific Primer Extension (ASPE) method. Expression of ADIPOR2 protein and mRNA in circulating CD14(+ )monocytes were determined using flow cytometry and real time Polymerase Chain Reaction assays respectively. RESULTS: There was a significant difference in the distribution of genotypes of polymorphism rs767870 of ADIPOR2 between CAD and non-CAD individuals (p = 0.017). Furthermore, heterozygotes of the rs767870 polymorphism had significantly lower Flow Mediated Dilatation (FMD) values, higher values of Intima-Media Thickness (IMT) and increased ADIPOR2 protein levels in peripheral monocytes, compared to homozygotes of the minor allele after adjustment for age, sex, waist to hip ratio and HOMA. CONCLUSIONS: Our findings suggest that variants of ADIPOR2 could be a determinant for atherosclerosis independent of insulin resistance status, possibly by affecting ADIPOR2 protein levels. BioMed Central 2010-02-23 /pmc/articles/PMC2846898/ /pubmed/20178558 http://dx.doi.org/10.1186/1475-2840-9-10 Text en Copyright ©2010 Halvatsiotis et al., licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original investigation Halvatsiotis, Iosif Tsiotra, Panayoula C Ikonomidis, Ignatios Kollias, Anastasios Mitrou, Panagiota Maratou, Eirini Boutati, Eleni Lekakis, John Dimitriadis, George Economopoulos, Theofanis Kremastinos, Dimitrios T Raptis, Sotirios A Genetic variation in the adiponectin receptor 2 (ADIPOR2) gene is associated with coronary artery disease and increased ADIPOR2 expression in peripheral monocytes |
title | Genetic variation in the adiponectin receptor 2 (ADIPOR2) gene is associated with coronary artery disease and increased ADIPOR2 expression in peripheral monocytes |
title_full | Genetic variation in the adiponectin receptor 2 (ADIPOR2) gene is associated with coronary artery disease and increased ADIPOR2 expression in peripheral monocytes |
title_fullStr | Genetic variation in the adiponectin receptor 2 (ADIPOR2) gene is associated with coronary artery disease and increased ADIPOR2 expression in peripheral monocytes |
title_full_unstemmed | Genetic variation in the adiponectin receptor 2 (ADIPOR2) gene is associated with coronary artery disease and increased ADIPOR2 expression in peripheral monocytes |
title_short | Genetic variation in the adiponectin receptor 2 (ADIPOR2) gene is associated with coronary artery disease and increased ADIPOR2 expression in peripheral monocytes |
title_sort | genetic variation in the adiponectin receptor 2 (adipor2) gene is associated with coronary artery disease and increased adipor2 expression in peripheral monocytes |
topic | Original investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2846898/ https://www.ncbi.nlm.nih.gov/pubmed/20178558 http://dx.doi.org/10.1186/1475-2840-9-10 |
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