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Flux of transcript patterns during soybean seed development

BACKGROUND: To understand gene expression networks leading to functional properties of the soybean seed, we have undertaken a detailed examination of soybean seed development during the stages of major accumulation of oils, proteins, and starches, as well as the desiccating and mature stages, using...

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Autores principales: Jones, Sarah I, Gonzalez, Delkin O, Vodkin, Lila O
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2846912/
https://www.ncbi.nlm.nih.gov/pubmed/20181280
http://dx.doi.org/10.1186/1471-2164-11-136
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author Jones, Sarah I
Gonzalez, Delkin O
Vodkin, Lila O
author_facet Jones, Sarah I
Gonzalez, Delkin O
Vodkin, Lila O
author_sort Jones, Sarah I
collection PubMed
description BACKGROUND: To understand gene expression networks leading to functional properties of the soybean seed, we have undertaken a detailed examination of soybean seed development during the stages of major accumulation of oils, proteins, and starches, as well as the desiccating and mature stages, using microarrays consisting of up to 27,000 soybean cDNAs. A subset of these genes on a highly-repetitive 70-mer oligonucleotide microarray was also used to support the results. RESULTS: It was discovered that genes related to cell growth and maintenance processes, as well as energy processes like photosynthesis, decreased in expression levels as the cotyledons approached the mature, dry stage. Genes involved with some storage proteins had their highest expression levels at the stage of highest fresh weight. However, genes encoding many transcription factors and DNA binding proteins showed higher expression levels in the desiccating and dry seeds than in most of the green stages. CONCLUSIONS: Data on 27,000 cDNAs have been obtained over five stages of soybean development, including the stages of major accumulation of agronomically-important products, using two different types of microarrays. Of particular interest are the genes found to peak in expression at the desiccating and dry seed stages, such as those annotated as transcription factors, which may indicate the preparation of pathways that will be needed later in the early stages of imbibition and germination.
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spelling pubmed-28469122010-03-30 Flux of transcript patterns during soybean seed development Jones, Sarah I Gonzalez, Delkin O Vodkin, Lila O BMC Genomics Research Article BACKGROUND: To understand gene expression networks leading to functional properties of the soybean seed, we have undertaken a detailed examination of soybean seed development during the stages of major accumulation of oils, proteins, and starches, as well as the desiccating and mature stages, using microarrays consisting of up to 27,000 soybean cDNAs. A subset of these genes on a highly-repetitive 70-mer oligonucleotide microarray was also used to support the results. RESULTS: It was discovered that genes related to cell growth and maintenance processes, as well as energy processes like photosynthesis, decreased in expression levels as the cotyledons approached the mature, dry stage. Genes involved with some storage proteins had their highest expression levels at the stage of highest fresh weight. However, genes encoding many transcription factors and DNA binding proteins showed higher expression levels in the desiccating and dry seeds than in most of the green stages. CONCLUSIONS: Data on 27,000 cDNAs have been obtained over five stages of soybean development, including the stages of major accumulation of agronomically-important products, using two different types of microarrays. Of particular interest are the genes found to peak in expression at the desiccating and dry seed stages, such as those annotated as transcription factors, which may indicate the preparation of pathways that will be needed later in the early stages of imbibition and germination. BioMed Central 2010-02-24 /pmc/articles/PMC2846912/ /pubmed/20181280 http://dx.doi.org/10.1186/1471-2164-11-136 Text en Copyright ©2010 Jones et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jones, Sarah I
Gonzalez, Delkin O
Vodkin, Lila O
Flux of transcript patterns during soybean seed development
title Flux of transcript patterns during soybean seed development
title_full Flux of transcript patterns during soybean seed development
title_fullStr Flux of transcript patterns during soybean seed development
title_full_unstemmed Flux of transcript patterns during soybean seed development
title_short Flux of transcript patterns during soybean seed development
title_sort flux of transcript patterns during soybean seed development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2846912/
https://www.ncbi.nlm.nih.gov/pubmed/20181280
http://dx.doi.org/10.1186/1471-2164-11-136
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