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Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria

IgG and IgG3 antibodies to merozoite surface protein-2 (MSP-2) of Plasmodium falciparum have been associated with protection from clinical malaria in independent studies. We determined whether this protection was allele-specific by testing whether children who developed clinical malaria lacked IgG/I...

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Autores principales: OSIER, F H A, MURUNGI, L M, FEGAN, G, TUJU, J, TETTEH, K K, BULL, P C, CONWAY, D J, MARSH, K
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847195/
https://www.ncbi.nlm.nih.gov/pubmed/20398182
http://dx.doi.org/10.1111/j.1365-3024.2009.01178.x
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author OSIER, F H A
MURUNGI, L M
FEGAN, G
TUJU, J
TETTEH, K K
BULL, P C
CONWAY, D J
MARSH, K
author_facet OSIER, F H A
MURUNGI, L M
FEGAN, G
TUJU, J
TETTEH, K K
BULL, P C
CONWAY, D J
MARSH, K
author_sort OSIER, F H A
collection PubMed
description IgG and IgG3 antibodies to merozoite surface protein-2 (MSP-2) of Plasmodium falciparum have been associated with protection from clinical malaria in independent studies. We determined whether this protection was allele-specific by testing whether children who developed clinical malaria lacked IgG/IgG3 antibodies specific to the dominant msp2 parasite genotypes detected during clinical episodes. We analysed pre-existing IgG and IgG1/IgG3 antibodies to antigens representing the major dimorphic types of MSP-2 by ELISA. We used quantitative real-time PCR to determine the dominant msp2 alleles in parasites detected in clinical episodes. Over half (55%, 80/146) of infections contained both allelic types. Single or dominant IC1- and FC27-like alleles were detected in 46% and 42% of infections respectively, and both types were equally dominant in 12%. High levels of IgG/IgG3 antibodies to the FC27-like antigen were not significantly associated with a lower likelihood of clinical episodes caused by parasites bearing FC27-like compared to IC1-like alleles, and vice versa for IgG/IgG3 antibodies to the IC1-like antigen. These findings were supported by competition ELISAs which demonstrated the presence of IgG antibodies to allele-specific epitopes within both antigens. Thus, even for this well-studied antigen, the importance of an allele-specific component of naturally acquired protective immunity to malaria remains to be confirmed.
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spelling pubmed-28471952010-04-08 Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria OSIER, F H A MURUNGI, L M FEGAN, G TUJU, J TETTEH, K K BULL, P C CONWAY, D J MARSH, K Parasite Immunol Original Articles IgG and IgG3 antibodies to merozoite surface protein-2 (MSP-2) of Plasmodium falciparum have been associated with protection from clinical malaria in independent studies. We determined whether this protection was allele-specific by testing whether children who developed clinical malaria lacked IgG/IgG3 antibodies specific to the dominant msp2 parasite genotypes detected during clinical episodes. We analysed pre-existing IgG and IgG1/IgG3 antibodies to antigens representing the major dimorphic types of MSP-2 by ELISA. We used quantitative real-time PCR to determine the dominant msp2 alleles in parasites detected in clinical episodes. Over half (55%, 80/146) of infections contained both allelic types. Single or dominant IC1- and FC27-like alleles were detected in 46% and 42% of infections respectively, and both types were equally dominant in 12%. High levels of IgG/IgG3 antibodies to the FC27-like antigen were not significantly associated with a lower likelihood of clinical episodes caused by parasites bearing FC27-like compared to IC1-like alleles, and vice versa for IgG/IgG3 antibodies to the IC1-like antigen. These findings were supported by competition ELISAs which demonstrated the presence of IgG antibodies to allele-specific epitopes within both antigens. Thus, even for this well-studied antigen, the importance of an allele-specific component of naturally acquired protective immunity to malaria remains to be confirmed. Blackwell Publishing Ltd 2010-03 /pmc/articles/PMC2847195/ /pubmed/20398182 http://dx.doi.org/10.1111/j.1365-3024.2009.01178.x Text en © 2010 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Original Articles
OSIER, F H A
MURUNGI, L M
FEGAN, G
TUJU, J
TETTEH, K K
BULL, P C
CONWAY, D J
MARSH, K
Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria
title Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria
title_full Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria
title_fullStr Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria
title_full_unstemmed Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria
title_short Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria
title_sort allele-specific antibodies to plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847195/
https://www.ncbi.nlm.nih.gov/pubmed/20398182
http://dx.doi.org/10.1111/j.1365-3024.2009.01178.x
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