The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant

A key step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate crossovers. Although the enzyme that performs this function in human cells is presently unknown, recent studies led to the identification of the XPG-family endonuclease GEN1 that promotes Hollid...

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Detalles Bibliográficos
Autores principales: Lorenz, Alexander, West, Stephen C., Whitby, Matthew C.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Genome Integrity, Repair and Replication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847240/
https://www.ncbi.nlm.nih.gov/pubmed/20040574
http://dx.doi.org/10.1093/nar/gkp1179
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author Lorenz, Alexander
West, Stephen C.
Whitby, Matthew C.
author_facet Lorenz, Alexander
West, Stephen C.
Whitby, Matthew C.
author_sort Lorenz, Alexander
collection PubMed
description A key step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate crossovers. Although the enzyme that performs this function in human cells is presently unknown, recent studies led to the identification of the XPG-family endonuclease GEN1 that promotes Holliday junction resolution in vitro, suggesting that it may perform a related function in vivo. Here, we show that ectopic expression of GEN1 in fission yeast mus81Δ strains results in Holliday junction resolution and crossover formation during meiosis.
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spelling pubmed-28472402010-04-01 The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant Lorenz, Alexander West, Stephen C. Whitby, Matthew C. Nucleic Acids Res Genome Integrity, Repair and Replication A key step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate crossovers. Although the enzyme that performs this function in human cells is presently unknown, recent studies led to the identification of the XPG-family endonuclease GEN1 that promotes Holliday junction resolution in vitro, suggesting that it may perform a related function in vivo. Here, we show that ectopic expression of GEN1 in fission yeast mus81Δ strains results in Holliday junction resolution and crossover formation during meiosis. Oxford University Press 2010-04 2009-12-29 /pmc/articles/PMC2847240/ /pubmed/20040574 http://dx.doi.org/10.1093/nar/gkp1179 Text en © The Author(s) 2009. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genome Integrity, Repair and Replication
Lorenz, Alexander
West, Stephen C.
Whitby, Matthew C.
The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant
title The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant
title_full The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant
title_fullStr The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant
title_full_unstemmed The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant
title_short The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant
title_sort human holliday junction resolvase gen1 rescues the meiotic phenotype of a schizosaccharomyces pombe mus81 mutant
topic Genome Integrity, Repair and Replication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847240/
https://www.ncbi.nlm.nih.gov/pubmed/20040574
http://dx.doi.org/10.1093/nar/gkp1179
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