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Optimization of the hybridization-based method for purification of thermostable tRNAs in the presence of tetraalkylammonium salts

We found that both tetramethylammonium chloride (TMA-Cl) and tetra-ethylammonium chloride (TEA-Cl), which are used as monovalent cations for northern hybridization, drastically destabilized the tertiary structures of tRNAs and enhanced the formation of tRNA•oligoDNA hybrids. These effects are of gre...

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Detalles Bibliográficos
Autores principales:	Yokogawa, Takashi, Kitamura, Yusuke, Nakamura, Daigo, Ohno, Satoshi, Nishikawa, Kazuya
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2010
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847242/ https://www.ncbi.nlm.nih.gov/pubmed/20040572 http://dx.doi.org/10.1093/nar/gkp1182

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