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Initial Polarized Bud Growth by Endocytic Recycling in the Absence of Actin Cable–dependent Vesicle Transport in Yeast

The assembly of filamentous actin is essential for polarized bud growth in budding yeast. Actin cables, which are assembled by the formins Bni1p and Bnr1p, are thought to be the only actin structures that are essential for budding. However, we found that formin or tropomyosin mutants, which lack act...

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Autores principales: Yamamoto, Takaharu, Mochida, Junko, Kadota, Jun, Takeda, Miyoko, Bi, Erfei, Tanaka, Kazuma
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847527/
https://www.ncbi.nlm.nih.gov/pubmed/20147449
http://dx.doi.org/10.1091/mbc.E09-05-0412
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author Yamamoto, Takaharu
Mochida, Junko
Kadota, Jun
Takeda, Miyoko
Bi, Erfei
Tanaka, Kazuma
author_facet Yamamoto, Takaharu
Mochida, Junko
Kadota, Jun
Takeda, Miyoko
Bi, Erfei
Tanaka, Kazuma
author_sort Yamamoto, Takaharu
collection PubMed
description The assembly of filamentous actin is essential for polarized bud growth in budding yeast. Actin cables, which are assembled by the formins Bni1p and Bnr1p, are thought to be the only actin structures that are essential for budding. However, we found that formin or tropomyosin mutants, which lack actin cables, are still able to form a small bud. Additional mutations in components for cortical actin patches, which are assembled by the Arp2/3 complex to play a pivotal role in endocytic vesicle formation, inhibited this budding. Genes involved in endocytic recycling were also required for small-bud formation in actin cable-less mutants. These results suggest that budding yeast possesses a mechanism that promotes polarized growth by local recycling of endocytic vesicles. Interestingly, the type V myosin Myo2p, which was thought to use only actin cables to track, also contributed to budding in the absence of actin cables. These results suggest that some actin network may serve as the track for Myo2p-driven vesicle transport in the absence of actin cables or that Myo2p can function independent of actin filaments. Our results also show that polarity regulators including Cdc42p were still polarized in mutants defective in both actin cables and cortical actin patches, suggesting that the actin cytoskeleton does not play a major role in cortical assembly of polarity regulators in budding yeast.
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spelling pubmed-28475272010-06-16 Initial Polarized Bud Growth by Endocytic Recycling in the Absence of Actin Cable–dependent Vesicle Transport in Yeast Yamamoto, Takaharu Mochida, Junko Kadota, Jun Takeda, Miyoko Bi, Erfei Tanaka, Kazuma Mol Biol Cell Articles The assembly of filamentous actin is essential for polarized bud growth in budding yeast. Actin cables, which are assembled by the formins Bni1p and Bnr1p, are thought to be the only actin structures that are essential for budding. However, we found that formin or tropomyosin mutants, which lack actin cables, are still able to form a small bud. Additional mutations in components for cortical actin patches, which are assembled by the Arp2/3 complex to play a pivotal role in endocytic vesicle formation, inhibited this budding. Genes involved in endocytic recycling were also required for small-bud formation in actin cable-less mutants. These results suggest that budding yeast possesses a mechanism that promotes polarized growth by local recycling of endocytic vesicles. Interestingly, the type V myosin Myo2p, which was thought to use only actin cables to track, also contributed to budding in the absence of actin cables. These results suggest that some actin network may serve as the track for Myo2p-driven vesicle transport in the absence of actin cables or that Myo2p can function independent of actin filaments. Our results also show that polarity regulators including Cdc42p were still polarized in mutants defective in both actin cables and cortical actin patches, suggesting that the actin cytoskeleton does not play a major role in cortical assembly of polarity regulators in budding yeast. The American Society for Cell Biology 2010-04-01 /pmc/articles/PMC2847527/ /pubmed/20147449 http://dx.doi.org/10.1091/mbc.E09-05-0412 Text en © 2010 by The American Society for Cell Biology
spellingShingle Articles
Yamamoto, Takaharu
Mochida, Junko
Kadota, Jun
Takeda, Miyoko
Bi, Erfei
Tanaka, Kazuma
Initial Polarized Bud Growth by Endocytic Recycling in the Absence of Actin Cable–dependent Vesicle Transport in Yeast
title Initial Polarized Bud Growth by Endocytic Recycling in the Absence of Actin Cable–dependent Vesicle Transport in Yeast
title_full Initial Polarized Bud Growth by Endocytic Recycling in the Absence of Actin Cable–dependent Vesicle Transport in Yeast
title_fullStr Initial Polarized Bud Growth by Endocytic Recycling in the Absence of Actin Cable–dependent Vesicle Transport in Yeast
title_full_unstemmed Initial Polarized Bud Growth by Endocytic Recycling in the Absence of Actin Cable–dependent Vesicle Transport in Yeast
title_short Initial Polarized Bud Growth by Endocytic Recycling in the Absence of Actin Cable–dependent Vesicle Transport in Yeast
title_sort initial polarized bud growth by endocytic recycling in the absence of actin cable–dependent vesicle transport in yeast
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847527/
https://www.ncbi.nlm.nih.gov/pubmed/20147449
http://dx.doi.org/10.1091/mbc.E09-05-0412
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