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Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast
BACKGROUND: In the picture of a laboratory evolution experiment, to improve the thermostability whilst maintaining the activity requires of suitable procedures to generate diversity in combination with robust high-throughput protocols. The current work describes how to achieve this goal by engineeri...
Autores principales: | García-Ruiz, Eva, Maté, Diana, Ballesteros, Antonio, Martinez, Angel T, Alcalde, Miguel |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847542/ https://www.ncbi.nlm.nih.gov/pubmed/20298573 http://dx.doi.org/10.1186/1475-2859-9-17 |
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