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A novel method for sample preparation of fresh lung cancer tissue for proteomics analysis by tumor cell enrichment and removal of blood contaminants
BACKGROUND: In-depth proteomics analyses of tumors are frequently biased by the presence of blood components and stromal contamination, which leads to large experimental variation and decreases the proteome coverage. We have established a reproducible method to prepare freshly collected lung tumors...
Autores principales: | De Petris, Luigi, Pernemalm, Maria, Elmberger, Göran, Bergman, Per, Orre, Lotta, Lewensohn, Rolf, Lehtiö, Janne |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847553/ https://www.ncbi.nlm.nih.gov/pubmed/20187940 http://dx.doi.org/10.1186/1477-5956-8-9 |
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