Cargando…
Quantification of Mineralized Bone Response to Prostate Cancer by Noninvasive In Vivo μCT and Non-Destructive Ex Vivo μCT and DXA in a Mouse Model
BACKGROUND: To compare nondestructive in vivo and ex vivo micro-computed tomography (μCT) and ex vivo dual-energy-X-ray-absorptiometry (DXA) in characterizing mineralized cortical and trabecular bone response to prostate cancer involving the skeleton in a mouse model. METHODOLOGY/PRINCIPAL FINDINGS:...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847943/ https://www.ncbi.nlm.nih.gov/pubmed/20360964 http://dx.doi.org/10.1371/journal.pone.0009854 |
_version_ | 1782179613807476736 |
---|---|
author | Ravoori, Murali Czaplinska, Aneta J. Sikes, Charles Han, Lin Johnson, Evan M. Qiao, Wei Ng, Chaan Cody, Dianna D. Murphy, William A. Do, Kim-Anh Navone, Nora M. Kundra, Vikas |
author_facet | Ravoori, Murali Czaplinska, Aneta J. Sikes, Charles Han, Lin Johnson, Evan M. Qiao, Wei Ng, Chaan Cody, Dianna D. Murphy, William A. Do, Kim-Anh Navone, Nora M. Kundra, Vikas |
author_sort | Ravoori, Murali |
collection | PubMed |
description | BACKGROUND: To compare nondestructive in vivo and ex vivo micro-computed tomography (μCT) and ex vivo dual-energy-X-ray-absorptiometry (DXA) in characterizing mineralized cortical and trabecular bone response to prostate cancer involving the skeleton in a mouse model. METHODOLOGY/PRINCIPAL FINDINGS: In vivo μCT was performed before and 10 weeks after implantation of human prostate cancer cells (MDA-PCa-2b) or vehicle into SCID mouse femora. After resection, femora were imaged by nondestructive ex vivo specimen μCT at three voxel sizes (31 µ, 16 µ, 8 µ) and DXA, and then sectioned for histomorphometric analysis of mineralized bone. Bone mineral density (BMD), trabecular parameters (number, TbN; separation, TbSp; thickness, TbTh) and mineralized bone volume/total bone volume (BV/TV) were compared and correlated among imaging methods and histomorphometry. Statistical tests were considered significant if P<0.05. Ten weeks post inoculation, diaphyseal BMD increased in the femur with tumor compared to the opposite femur by all modalities (p<0.005, n = 11). Diaphyseal BMD by in vivo μCT correlated with ex vivo 31 and 16 µm μCT and histomorphometry BV/TV (r = 0.91–0.94, P<0.001, n = 11). DXA BMD correlated less with bone histomorphometry (r = 0.73, P<0.001, n = 11) and DXA did not distinguish trabeculae from cortex. By in vivo and ex vivo μCT, trabecular BMD decreased (P<0.05, n = 11) as opposed to the cortex. Unlike BMD, trabecular morphologic parameters were threshold-dependent and when using “fixed-optimal-thresholds,” all except TbTh demonstrated trabecular loss with tumor and correlated with histomorphometry (r = 0.73–0.90, P<0.05, n = 11). CONCLUSIONS/SIGNIFICANCE: Prostate cancer involving the skeleton can elicit a host bone response that differentially affects the cortex compared to trabeculae and that can be quantified noninvasively in vivo and nondestructively ex vivo. |
format | Text |
id | pubmed-2847943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28479432010-04-01 Quantification of Mineralized Bone Response to Prostate Cancer by Noninvasive In Vivo μCT and Non-Destructive Ex Vivo μCT and DXA in a Mouse Model Ravoori, Murali Czaplinska, Aneta J. Sikes, Charles Han, Lin Johnson, Evan M. Qiao, Wei Ng, Chaan Cody, Dianna D. Murphy, William A. Do, Kim-Anh Navone, Nora M. Kundra, Vikas PLoS One Research Article BACKGROUND: To compare nondestructive in vivo and ex vivo micro-computed tomography (μCT) and ex vivo dual-energy-X-ray-absorptiometry (DXA) in characterizing mineralized cortical and trabecular bone response to prostate cancer involving the skeleton in a mouse model. METHODOLOGY/PRINCIPAL FINDINGS: In vivo μCT was performed before and 10 weeks after implantation of human prostate cancer cells (MDA-PCa-2b) or vehicle into SCID mouse femora. After resection, femora were imaged by nondestructive ex vivo specimen μCT at three voxel sizes (31 µ, 16 µ, 8 µ) and DXA, and then sectioned for histomorphometric analysis of mineralized bone. Bone mineral density (BMD), trabecular parameters (number, TbN; separation, TbSp; thickness, TbTh) and mineralized bone volume/total bone volume (BV/TV) were compared and correlated among imaging methods and histomorphometry. Statistical tests were considered significant if P<0.05. Ten weeks post inoculation, diaphyseal BMD increased in the femur with tumor compared to the opposite femur by all modalities (p<0.005, n = 11). Diaphyseal BMD by in vivo μCT correlated with ex vivo 31 and 16 µm μCT and histomorphometry BV/TV (r = 0.91–0.94, P<0.001, n = 11). DXA BMD correlated less with bone histomorphometry (r = 0.73, P<0.001, n = 11) and DXA did not distinguish trabeculae from cortex. By in vivo and ex vivo μCT, trabecular BMD decreased (P<0.05, n = 11) as opposed to the cortex. Unlike BMD, trabecular morphologic parameters were threshold-dependent and when using “fixed-optimal-thresholds,” all except TbTh demonstrated trabecular loss with tumor and correlated with histomorphometry (r = 0.73–0.90, P<0.05, n = 11). CONCLUSIONS/SIGNIFICANCE: Prostate cancer involving the skeleton can elicit a host bone response that differentially affects the cortex compared to trabeculae and that can be quantified noninvasively in vivo and nondestructively ex vivo. Public Library of Science 2010-03-29 /pmc/articles/PMC2847943/ /pubmed/20360964 http://dx.doi.org/10.1371/journal.pone.0009854 Text en Ravoori et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ravoori, Murali Czaplinska, Aneta J. Sikes, Charles Han, Lin Johnson, Evan M. Qiao, Wei Ng, Chaan Cody, Dianna D. Murphy, William A. Do, Kim-Anh Navone, Nora M. Kundra, Vikas Quantification of Mineralized Bone Response to Prostate Cancer by Noninvasive In Vivo μCT and Non-Destructive Ex Vivo μCT and DXA in a Mouse Model |
title | Quantification of Mineralized Bone Response to Prostate Cancer by Noninvasive In Vivo μCT and Non-Destructive Ex Vivo μCT and DXA in a Mouse Model |
title_full | Quantification of Mineralized Bone Response to Prostate Cancer by Noninvasive In Vivo μCT and Non-Destructive Ex Vivo μCT and DXA in a Mouse Model |
title_fullStr | Quantification of Mineralized Bone Response to Prostate Cancer by Noninvasive In Vivo μCT and Non-Destructive Ex Vivo μCT and DXA in a Mouse Model |
title_full_unstemmed | Quantification of Mineralized Bone Response to Prostate Cancer by Noninvasive In Vivo μCT and Non-Destructive Ex Vivo μCT and DXA in a Mouse Model |
title_short | Quantification of Mineralized Bone Response to Prostate Cancer by Noninvasive In Vivo μCT and Non-Destructive Ex Vivo μCT and DXA in a Mouse Model |
title_sort | quantification of mineralized bone response to prostate cancer by noninvasive in vivo μct and non-destructive ex vivo μct and dxa in a mouse model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847943/ https://www.ncbi.nlm.nih.gov/pubmed/20360964 http://dx.doi.org/10.1371/journal.pone.0009854 |
work_keys_str_mv | AT ravoorimurali quantificationofmineralizedboneresponsetoprostatecancerbynoninvasiveinvivomctandnondestructiveexvivomctanddxainamousemodel AT czaplinskaanetaj quantificationofmineralizedboneresponsetoprostatecancerbynoninvasiveinvivomctandnondestructiveexvivomctanddxainamousemodel AT sikescharles quantificationofmineralizedboneresponsetoprostatecancerbynoninvasiveinvivomctandnondestructiveexvivomctanddxainamousemodel AT hanlin quantificationofmineralizedboneresponsetoprostatecancerbynoninvasiveinvivomctandnondestructiveexvivomctanddxainamousemodel AT johnsonevanm quantificationofmineralizedboneresponsetoprostatecancerbynoninvasiveinvivomctandnondestructiveexvivomctanddxainamousemodel AT qiaowei quantificationofmineralizedboneresponsetoprostatecancerbynoninvasiveinvivomctandnondestructiveexvivomctanddxainamousemodel AT ngchaan quantificationofmineralizedboneresponsetoprostatecancerbynoninvasiveinvivomctandnondestructiveexvivomctanddxainamousemodel AT codydiannad quantificationofmineralizedboneresponsetoprostatecancerbynoninvasiveinvivomctandnondestructiveexvivomctanddxainamousemodel AT murphywilliama quantificationofmineralizedboneresponsetoprostatecancerbynoninvasiveinvivomctandnondestructiveexvivomctanddxainamousemodel AT dokimanh quantificationofmineralizedboneresponsetoprostatecancerbynoninvasiveinvivomctandnondestructiveexvivomctanddxainamousemodel AT navonenoram quantificationofmineralizedboneresponsetoprostatecancerbynoninvasiveinvivomctandnondestructiveexvivomctanddxainamousemodel AT kundravikas quantificationofmineralizedboneresponsetoprostatecancerbynoninvasiveinvivomctandnondestructiveexvivomctanddxainamousemodel |