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A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering
BACKGROUND: The combined use of restriction enzymes with PCR has revolutionized molecular cloning, but is inherently restricted by the content of the manipulated DNA sequences. Uracil-excision based cloning is ligase and sequence independent and allows seamless fusion of multiple DNA sequences in si...
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| Formato: | Texto |
| Lenguaje: | English |
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BioMed Central
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847956/ https://www.ncbi.nlm.nih.gov/pubmed/20233396 http://dx.doi.org/10.1186/1472-6750-10-21 |