Cargando…
Pepper Mild Mottle Virus, a Plant Virus Associated with Specific Immune Responses, Fever, Abdominal Pains, and Pruritus in Humans
BACKGROUND: Recently, metagenomic studies have identified viable Pepper mild mottle virus (PMMoV), a plant virus, in the stool of healthy subjects. However, its source and role as pathogen have not been determined. METHODS AND FINDINGS: 21 commercialized food products containing peppers, 357 stool s...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2850318/ https://www.ncbi.nlm.nih.gov/pubmed/20386604 http://dx.doi.org/10.1371/journal.pone.0010041 |
_version_ | 1782179767216242688 |
---|---|
author | Colson, Philippe Richet, Hervé Desnues, Christelle Balique, Fanny Moal, Valérie Grob, Jean-Jacques Berbis, Philippe Lecoq, Hervé Harlé, Jean-Robert Berland, Yvon Raoult, Didier |
author_facet | Colson, Philippe Richet, Hervé Desnues, Christelle Balique, Fanny Moal, Valérie Grob, Jean-Jacques Berbis, Philippe Lecoq, Hervé Harlé, Jean-Robert Berland, Yvon Raoult, Didier |
author_sort | Colson, Philippe |
collection | PubMed |
description | BACKGROUND: Recently, metagenomic studies have identified viable Pepper mild mottle virus (PMMoV), a plant virus, in the stool of healthy subjects. However, its source and role as pathogen have not been determined. METHODS AND FINDINGS: 21 commercialized food products containing peppers, 357 stool samples from 304 adults and 208 stool samples from 137 children were tested for PMMoV using real-time PCR, sequencing, and electron microscopy. Anti-PMMoV IgM antibody testing was concurrently performed. A case-control study tested the association of biological and clinical symptoms with the presence of PMMoV in the stool. Twelve (57%) food products were positive for PMMoV RNA sequencing. Stool samples from twenty-two (7.2%) adults and one child (0.7%) were positive for PMMoV by real-time PCR. Positive cases were significantly more likely to have been sampled in Dermatology Units (p<10(−6)), to be seropositive for anti-PMMoV IgM antibodies (p = 0.026) and to be patients who exhibited fever, abdominal pains, and pruritus (p = 0.045, 0.038 and 0.046, respectively). CONCLUSIONS: Our study identified a local source of PMMoV and linked the presence of PMMoV RNA in stool with a specific immune response and clinical symptoms. Although clinical symptoms may be imputable to another cofactor, including spicy food, our data suggest the possibility of a direct or indirect pathogenic role of plant viruses in humans. |
format | Text |
id | pubmed-2850318 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28503182010-04-12 Pepper Mild Mottle Virus, a Plant Virus Associated with Specific Immune Responses, Fever, Abdominal Pains, and Pruritus in Humans Colson, Philippe Richet, Hervé Desnues, Christelle Balique, Fanny Moal, Valérie Grob, Jean-Jacques Berbis, Philippe Lecoq, Hervé Harlé, Jean-Robert Berland, Yvon Raoult, Didier PLoS One Research Article BACKGROUND: Recently, metagenomic studies have identified viable Pepper mild mottle virus (PMMoV), a plant virus, in the stool of healthy subjects. However, its source and role as pathogen have not been determined. METHODS AND FINDINGS: 21 commercialized food products containing peppers, 357 stool samples from 304 adults and 208 stool samples from 137 children were tested for PMMoV using real-time PCR, sequencing, and electron microscopy. Anti-PMMoV IgM antibody testing was concurrently performed. A case-control study tested the association of biological and clinical symptoms with the presence of PMMoV in the stool. Twelve (57%) food products were positive for PMMoV RNA sequencing. Stool samples from twenty-two (7.2%) adults and one child (0.7%) were positive for PMMoV by real-time PCR. Positive cases were significantly more likely to have been sampled in Dermatology Units (p<10(−6)), to be seropositive for anti-PMMoV IgM antibodies (p = 0.026) and to be patients who exhibited fever, abdominal pains, and pruritus (p = 0.045, 0.038 and 0.046, respectively). CONCLUSIONS: Our study identified a local source of PMMoV and linked the presence of PMMoV RNA in stool with a specific immune response and clinical symptoms. Although clinical symptoms may be imputable to another cofactor, including spicy food, our data suggest the possibility of a direct or indirect pathogenic role of plant viruses in humans. Public Library of Science 2010-04-06 /pmc/articles/PMC2850318/ /pubmed/20386604 http://dx.doi.org/10.1371/journal.pone.0010041 Text en Colson et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Colson, Philippe Richet, Hervé Desnues, Christelle Balique, Fanny Moal, Valérie Grob, Jean-Jacques Berbis, Philippe Lecoq, Hervé Harlé, Jean-Robert Berland, Yvon Raoult, Didier Pepper Mild Mottle Virus, a Plant Virus Associated with Specific Immune Responses, Fever, Abdominal Pains, and Pruritus in Humans |
title | Pepper Mild Mottle Virus, a Plant Virus Associated with Specific Immune Responses, Fever, Abdominal Pains, and Pruritus in Humans |
title_full | Pepper Mild Mottle Virus, a Plant Virus Associated with Specific Immune Responses, Fever, Abdominal Pains, and Pruritus in Humans |
title_fullStr | Pepper Mild Mottle Virus, a Plant Virus Associated with Specific Immune Responses, Fever, Abdominal Pains, and Pruritus in Humans |
title_full_unstemmed | Pepper Mild Mottle Virus, a Plant Virus Associated with Specific Immune Responses, Fever, Abdominal Pains, and Pruritus in Humans |
title_short | Pepper Mild Mottle Virus, a Plant Virus Associated with Specific Immune Responses, Fever, Abdominal Pains, and Pruritus in Humans |
title_sort | pepper mild mottle virus, a plant virus associated with specific immune responses, fever, abdominal pains, and pruritus in humans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2850318/ https://www.ncbi.nlm.nih.gov/pubmed/20386604 http://dx.doi.org/10.1371/journal.pone.0010041 |
work_keys_str_mv | AT colsonphilippe peppermildmottlevirusaplantvirusassociatedwithspecificimmuneresponsesfeverabdominalpainsandpruritusinhumans AT richetherve peppermildmottlevirusaplantvirusassociatedwithspecificimmuneresponsesfeverabdominalpainsandpruritusinhumans AT desnueschristelle peppermildmottlevirusaplantvirusassociatedwithspecificimmuneresponsesfeverabdominalpainsandpruritusinhumans AT baliquefanny peppermildmottlevirusaplantvirusassociatedwithspecificimmuneresponsesfeverabdominalpainsandpruritusinhumans AT moalvalerie peppermildmottlevirusaplantvirusassociatedwithspecificimmuneresponsesfeverabdominalpainsandpruritusinhumans AT grobjeanjacques peppermildmottlevirusaplantvirusassociatedwithspecificimmuneresponsesfeverabdominalpainsandpruritusinhumans AT berbisphilippe peppermildmottlevirusaplantvirusassociatedwithspecificimmuneresponsesfeverabdominalpainsandpruritusinhumans AT lecoqherve peppermildmottlevirusaplantvirusassociatedwithspecificimmuneresponsesfeverabdominalpainsandpruritusinhumans AT harlejeanrobert peppermildmottlevirusaplantvirusassociatedwithspecificimmuneresponsesfeverabdominalpainsandpruritusinhumans AT berlandyvon peppermildmottlevirusaplantvirusassociatedwithspecificimmuneresponsesfeverabdominalpainsandpruritusinhumans AT raoultdidier peppermildmottlevirusaplantvirusassociatedwithspecificimmuneresponsesfeverabdominalpainsandpruritusinhumans |