Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions

We describe the use of site specific recombination to study the host-parasite interactions of Toxoplasma gondii. We present a Toxoplasma strain that efficiently injects a Cre fusion protein into host cells. In a Cre-reporter cell line, a single parasite invasion induced Cre-mediated recombination in...

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Detalles Bibliográficos
Autores principales: Koshy, Anita A., Fouts, Ashley E., Lodoen, Melissa B., Alkan, Ozan, Blau, Helen M., Boothroyd, John C.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2850821/
https://www.ncbi.nlm.nih.gov/pubmed/20208532
http://dx.doi.org/10.1038/nmeth.1438
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author Koshy, Anita A.
Fouts, Ashley E.
Lodoen, Melissa B.
Alkan, Ozan
Blau, Helen M.
Boothroyd, John C.
author_facet Koshy, Anita A.
Fouts, Ashley E.
Lodoen, Melissa B.
Alkan, Ozan
Blau, Helen M.
Boothroyd, John C.
author_sort Koshy, Anita A.
collection PubMed
description We describe the use of site specific recombination to study the host-parasite interactions of Toxoplasma gondii. We present a Toxoplasma strain that efficiently injects a Cre fusion protein into host cells. In a Cre-reporter cell line, a single parasite invasion induced Cre-mediated recombination in 95% of infected host cells. By infecting Cre reporter mice with these parasites, host cell infection could also be monitored in vivo.
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spelling pubmed-28508212010-10-01 Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions Koshy, Anita A. Fouts, Ashley E. Lodoen, Melissa B. Alkan, Ozan Blau, Helen M. Boothroyd, John C. Nat Methods Article We describe the use of site specific recombination to study the host-parasite interactions of Toxoplasma gondii. We present a Toxoplasma strain that efficiently injects a Cre fusion protein into host cells. In a Cre-reporter cell line, a single parasite invasion induced Cre-mediated recombination in 95% of infected host cells. By infecting Cre reporter mice with these parasites, host cell infection could also be monitored in vivo. 2010-03-07 2010-04 /pmc/articles/PMC2850821/ /pubmed/20208532 http://dx.doi.org/10.1038/nmeth.1438 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Koshy, Anita A.
Fouts, Ashley E.
Lodoen, Melissa B.
Alkan, Ozan
Blau, Helen M.
Boothroyd, John C.
Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions
title Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions
title_full Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions
title_fullStr Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions
title_full_unstemmed Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions
title_short Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions
title_sort toxoplasma secreting cre recombinase for analysis of host-parasite interactions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2850821/
https://www.ncbi.nlm.nih.gov/pubmed/20208532
http://dx.doi.org/10.1038/nmeth.1438
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