Cargando…
Aquaporin expression in blood-retinal barrier cells during experimental autoimmune uveitis
PURPOSE: Blood-retinal barrier (BRB) breakdown and retinal edema are major complications of autoimmune uveitis and could be related to deregulation of aquaporin (AQP) expression. We have therefore evaluated the expression of AQP1 and AQP4 on BRB cells during experimental autoimmune uveitis (EAU) in...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2850934/ https://www.ncbi.nlm.nih.gov/pubmed/20383338 |
_version_ | 1782179825597808640 |
---|---|
author | Motulsky, Elie Koch, Philippe Janssens, Sarah Liénart, Maité Vanbellinghen, Anne-Marie Bolaky, Nargis Chan, Chi-Chao Caspers, Laure Martin-Martinez, Maria-Dolores Xu, Heping Delporte, Christine Willermain, François |
author_facet | Motulsky, Elie Koch, Philippe Janssens, Sarah Liénart, Maité Vanbellinghen, Anne-Marie Bolaky, Nargis Chan, Chi-Chao Caspers, Laure Martin-Martinez, Maria-Dolores Xu, Heping Delporte, Christine Willermain, François |
author_sort | Motulsky, Elie |
collection | PubMed |
description | PURPOSE: Blood-retinal barrier (BRB) breakdown and retinal edema are major complications of autoimmune uveitis and could be related to deregulation of aquaporin (AQP) expression. We have therefore evaluated the expression of AQP1 and AQP4 on BRB cells during experimental autoimmune uveitis (EAU) in mice. METHODS: C57Bl6 mice were immunized with interphotoreceptor retinoid-binding protein (IRBP) peptide 1–16. The disease was graded clinically, and double immunolabeling using glial fibrillary acidic protein (GFAP; a marker of disease activity) and AQP1 or AQP4 antibodies was performed at day 28. AQP1 expression was also investigated in mouse retinal pigment epithelium (RPE) cells (B6-RPE07 cell line) by reverse transcriptase PCR and western blot under basal and tumor necrosis factor α (TNF-α)-stimulated conditions. RESULTS: In both normal and EAU retina, AQP1 and AQP4 expression were restricted to the photoreceptor layer and to the Müller cells, respectively. Retinal endothelial cells never expressed AQP1. In vasculitis and intraretinal inflammatory infiltrates, decreased AQP1 expression was observed due to the loss of photoreceptors and the characteristic radial labeling of AQP4 was lost. On the other hand, no AQP4 expression was detected in RPE cells. AQP1 was strongly expressed by choroidal endothelial cells, rendering difficult the evaluation of AQP1 expression by RPE cells in vivo. No major differences were found between EAU and controls at this level. Interestingly, B6-RPE07 cells expressed AQP1 in vitro, and TNF-α downregulated AQP1 protein expression in those cells. CONCLUSIONS: Changes in retinal expression of AQP1 and AQP4 during EAU were primarily due to inflammatory lesions, contrasting with major modulation of AQP expression in BRB detected in other models of BRB breakdown. However, our data showed that TNF-α treatment strongly modulates AQP1 expression in B6-RPE07 cells in vitro. |
format | Text |
id | pubmed-2850934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-28509342010-04-09 Aquaporin expression in blood-retinal barrier cells during experimental autoimmune uveitis Motulsky, Elie Koch, Philippe Janssens, Sarah Liénart, Maité Vanbellinghen, Anne-Marie Bolaky, Nargis Chan, Chi-Chao Caspers, Laure Martin-Martinez, Maria-Dolores Xu, Heping Delporte, Christine Willermain, François Mol Vis Research Article PURPOSE: Blood-retinal barrier (BRB) breakdown and retinal edema are major complications of autoimmune uveitis and could be related to deregulation of aquaporin (AQP) expression. We have therefore evaluated the expression of AQP1 and AQP4 on BRB cells during experimental autoimmune uveitis (EAU) in mice. METHODS: C57Bl6 mice were immunized with interphotoreceptor retinoid-binding protein (IRBP) peptide 1–16. The disease was graded clinically, and double immunolabeling using glial fibrillary acidic protein (GFAP; a marker of disease activity) and AQP1 or AQP4 antibodies was performed at day 28. AQP1 expression was also investigated in mouse retinal pigment epithelium (RPE) cells (B6-RPE07 cell line) by reverse transcriptase PCR and western blot under basal and tumor necrosis factor α (TNF-α)-stimulated conditions. RESULTS: In both normal and EAU retina, AQP1 and AQP4 expression were restricted to the photoreceptor layer and to the Müller cells, respectively. Retinal endothelial cells never expressed AQP1. In vasculitis and intraretinal inflammatory infiltrates, decreased AQP1 expression was observed due to the loss of photoreceptors and the characteristic radial labeling of AQP4 was lost. On the other hand, no AQP4 expression was detected in RPE cells. AQP1 was strongly expressed by choroidal endothelial cells, rendering difficult the evaluation of AQP1 expression by RPE cells in vivo. No major differences were found between EAU and controls at this level. Interestingly, B6-RPE07 cells expressed AQP1 in vitro, and TNF-α downregulated AQP1 protein expression in those cells. CONCLUSIONS: Changes in retinal expression of AQP1 and AQP4 during EAU were primarily due to inflammatory lesions, contrasting with major modulation of AQP expression in BRB detected in other models of BRB breakdown. However, our data showed that TNF-α treatment strongly modulates AQP1 expression in B6-RPE07 cells in vitro. Molecular Vision 2010-04-03 /pmc/articles/PMC2850934/ /pubmed/20383338 Text en Copyright © 2010 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Motulsky, Elie Koch, Philippe Janssens, Sarah Liénart, Maité Vanbellinghen, Anne-Marie Bolaky, Nargis Chan, Chi-Chao Caspers, Laure Martin-Martinez, Maria-Dolores Xu, Heping Delporte, Christine Willermain, François Aquaporin expression in blood-retinal barrier cells during experimental autoimmune uveitis |
title | Aquaporin expression in blood-retinal barrier cells during experimental autoimmune uveitis |
title_full | Aquaporin expression in blood-retinal barrier cells during experimental autoimmune uveitis |
title_fullStr | Aquaporin expression in blood-retinal barrier cells during experimental autoimmune uveitis |
title_full_unstemmed | Aquaporin expression in blood-retinal barrier cells during experimental autoimmune uveitis |
title_short | Aquaporin expression in blood-retinal barrier cells during experimental autoimmune uveitis |
title_sort | aquaporin expression in blood-retinal barrier cells during experimental autoimmune uveitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2850934/ https://www.ncbi.nlm.nih.gov/pubmed/20383338 |
work_keys_str_mv | AT motulskyelie aquaporinexpressioninbloodretinalbarriercellsduringexperimentalautoimmuneuveitis AT kochphilippe aquaporinexpressioninbloodretinalbarriercellsduringexperimentalautoimmuneuveitis AT janssenssarah aquaporinexpressioninbloodretinalbarriercellsduringexperimentalautoimmuneuveitis AT lienartmaite aquaporinexpressioninbloodretinalbarriercellsduringexperimentalautoimmuneuveitis AT vanbellinghenannemarie aquaporinexpressioninbloodretinalbarriercellsduringexperimentalautoimmuneuveitis AT bolakynargis aquaporinexpressioninbloodretinalbarriercellsduringexperimentalautoimmuneuveitis AT chanchichao aquaporinexpressioninbloodretinalbarriercellsduringexperimentalautoimmuneuveitis AT casperslaure aquaporinexpressioninbloodretinalbarriercellsduringexperimentalautoimmuneuveitis AT martinmartinezmariadolores aquaporinexpressioninbloodretinalbarriercellsduringexperimentalautoimmuneuveitis AT xuheping aquaporinexpressioninbloodretinalbarriercellsduringexperimentalautoimmuneuveitis AT delportechristine aquaporinexpressioninbloodretinalbarriercellsduringexperimentalautoimmuneuveitis AT willermainfrancois aquaporinexpressioninbloodretinalbarriercellsduringexperimentalautoimmuneuveitis |