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Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin

A buccoadhesive drug delivery system of Insulin was prepared by solvent casting technique and characterized in vitro by surface pH, bioadhesive strength, drug release and skin permeation studies. Sodium carboxymethylcellulose-DVP was chosen as the controlled release matrix polymer. The optimized for...

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Autores principales: Sahni, J., Raj, S., Ahmad, F. J., Khar, R. K.
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852063/
https://www.ncbi.nlm.nih.gov/pubmed/20390082
http://dx.doi.org/10.4103/0250-474X.40333
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author Sahni, J.
Raj, S.
Ahmad, F. J.
Khar, R. K.
author_facet Sahni, J.
Raj, S.
Ahmad, F. J.
Khar, R. K.
author_sort Sahni, J.
collection PubMed
description A buccoadhesive drug delivery system of Insulin was prepared by solvent casting technique and characterized in vitro by surface pH, bioadhesive strength, drug release and skin permeation studies. Sodium carboxymethylcellulose-DVP was chosen as the controlled release matrix polymer. The optimized formulation J(4) contained Sodium carboxy methyl cellulose-DVP 2% (w/v), insulin (50 IU/film), propylene glycol (0.25 ml) and Isopropyl alcohol: water (1:4) as solvent system. Bioadhesive strength of the prepared patches was measured on a modified physical balance using bovine cheek pouch as the model membrane. In vitro release studies were carried out at 37 ± 2° using phosphate buffer pH 6.6, in a modified dissolution apparatus fabricated for the purpose. Cumulative amount of drug released from the optimized formulation J(4) was 91.64% in 6 hours. In vitro permeation studies were carried out on J(4) at 37 ± 2° using Franz diffusion cell. Cumulative amount of drug permeated from J(4) was 6.63% in 6 hours. In order to enhance the permeation of protein drug, different permeation enhancers were evaluated. The results suggested that sodium deoxycholate 5% (w/v) was the best permeation enhancer among those evaluated. It enhanced the permeation of insulin from 6.63% to 10.38% over a period of 6 hours. The optimized patches were also satisfactory in terms of surface pH and bioadhesive strength. It can also be easily concluded that the system is a success as compared to the conventional formulations with respect to invasiveness, requirement of trained persons for administration and most importantly, the first pass metabolism.
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spelling pubmed-28520632010-04-13 Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin Sahni, J. Raj, S. Ahmad, F. J. Khar, R. K. Indian J Pharm Sci Research Paper A buccoadhesive drug delivery system of Insulin was prepared by solvent casting technique and characterized in vitro by surface pH, bioadhesive strength, drug release and skin permeation studies. Sodium carboxymethylcellulose-DVP was chosen as the controlled release matrix polymer. The optimized formulation J(4) contained Sodium carboxy methyl cellulose-DVP 2% (w/v), insulin (50 IU/film), propylene glycol (0.25 ml) and Isopropyl alcohol: water (1:4) as solvent system. Bioadhesive strength of the prepared patches was measured on a modified physical balance using bovine cheek pouch as the model membrane. In vitro release studies were carried out at 37 ± 2° using phosphate buffer pH 6.6, in a modified dissolution apparatus fabricated for the purpose. Cumulative amount of drug released from the optimized formulation J(4) was 91.64% in 6 hours. In vitro permeation studies were carried out on J(4) at 37 ± 2° using Franz diffusion cell. Cumulative amount of drug permeated from J(4) was 6.63% in 6 hours. In order to enhance the permeation of protein drug, different permeation enhancers were evaluated. The results suggested that sodium deoxycholate 5% (w/v) was the best permeation enhancer among those evaluated. It enhanced the permeation of insulin from 6.63% to 10.38% over a period of 6 hours. The optimized patches were also satisfactory in terms of surface pH and bioadhesive strength. It can also be easily concluded that the system is a success as compared to the conventional formulations with respect to invasiveness, requirement of trained persons for administration and most importantly, the first pass metabolism. Medknow Publications 2008 /pmc/articles/PMC2852063/ /pubmed/20390082 http://dx.doi.org/10.4103/0250-474X.40333 Text en © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Sahni, J.
Raj, S.
Ahmad, F. J.
Khar, R. K.
Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin
title Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin
title_full Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin
title_fullStr Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin
title_full_unstemmed Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin
title_short Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin
title_sort design and in vitro characterization of buccoadhesive drug delivery system of insulin
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852063/
https://www.ncbi.nlm.nih.gov/pubmed/20390082
http://dx.doi.org/10.4103/0250-474X.40333
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