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Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin
A buccoadhesive drug delivery system of Insulin was prepared by solvent casting technique and characterized in vitro by surface pH, bioadhesive strength, drug release and skin permeation studies. Sodium carboxymethylcellulose-DVP was chosen as the controlled release matrix polymer. The optimized for...
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Formato: | Texto |
Lenguaje: | English |
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Medknow Publications
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852063/ https://www.ncbi.nlm.nih.gov/pubmed/20390082 http://dx.doi.org/10.4103/0250-474X.40333 |
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author | Sahni, J. Raj, S. Ahmad, F. J. Khar, R. K. |
author_facet | Sahni, J. Raj, S. Ahmad, F. J. Khar, R. K. |
author_sort | Sahni, J. |
collection | PubMed |
description | A buccoadhesive drug delivery system of Insulin was prepared by solvent casting technique and characterized in vitro by surface pH, bioadhesive strength, drug release and skin permeation studies. Sodium carboxymethylcellulose-DVP was chosen as the controlled release matrix polymer. The optimized formulation J(4) contained Sodium carboxy methyl cellulose-DVP 2% (w/v), insulin (50 IU/film), propylene glycol (0.25 ml) and Isopropyl alcohol: water (1:4) as solvent system. Bioadhesive strength of the prepared patches was measured on a modified physical balance using bovine cheek pouch as the model membrane. In vitro release studies were carried out at 37 ± 2° using phosphate buffer pH 6.6, in a modified dissolution apparatus fabricated for the purpose. Cumulative amount of drug released from the optimized formulation J(4) was 91.64% in 6 hours. In vitro permeation studies were carried out on J(4) at 37 ± 2° using Franz diffusion cell. Cumulative amount of drug permeated from J(4) was 6.63% in 6 hours. In order to enhance the permeation of protein drug, different permeation enhancers were evaluated. The results suggested that sodium deoxycholate 5% (w/v) was the best permeation enhancer among those evaluated. It enhanced the permeation of insulin from 6.63% to 10.38% over a period of 6 hours. The optimized patches were also satisfactory in terms of surface pH and bioadhesive strength. It can also be easily concluded that the system is a success as compared to the conventional formulations with respect to invasiveness, requirement of trained persons for administration and most importantly, the first pass metabolism. |
format | Text |
id | pubmed-2852063 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Medknow Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-28520632010-04-13 Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin Sahni, J. Raj, S. Ahmad, F. J. Khar, R. K. Indian J Pharm Sci Research Paper A buccoadhesive drug delivery system of Insulin was prepared by solvent casting technique and characterized in vitro by surface pH, bioadhesive strength, drug release and skin permeation studies. Sodium carboxymethylcellulose-DVP was chosen as the controlled release matrix polymer. The optimized formulation J(4) contained Sodium carboxy methyl cellulose-DVP 2% (w/v), insulin (50 IU/film), propylene glycol (0.25 ml) and Isopropyl alcohol: water (1:4) as solvent system. Bioadhesive strength of the prepared patches was measured on a modified physical balance using bovine cheek pouch as the model membrane. In vitro release studies were carried out at 37 ± 2° using phosphate buffer pH 6.6, in a modified dissolution apparatus fabricated for the purpose. Cumulative amount of drug released from the optimized formulation J(4) was 91.64% in 6 hours. In vitro permeation studies were carried out on J(4) at 37 ± 2° using Franz diffusion cell. Cumulative amount of drug permeated from J(4) was 6.63% in 6 hours. In order to enhance the permeation of protein drug, different permeation enhancers were evaluated. The results suggested that sodium deoxycholate 5% (w/v) was the best permeation enhancer among those evaluated. It enhanced the permeation of insulin from 6.63% to 10.38% over a period of 6 hours. The optimized patches were also satisfactory in terms of surface pH and bioadhesive strength. It can also be easily concluded that the system is a success as compared to the conventional formulations with respect to invasiveness, requirement of trained persons for administration and most importantly, the first pass metabolism. Medknow Publications 2008 /pmc/articles/PMC2852063/ /pubmed/20390082 http://dx.doi.org/10.4103/0250-474X.40333 Text en © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Sahni, J. Raj, S. Ahmad, F. J. Khar, R. K. Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin |
title | Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin |
title_full | Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin |
title_fullStr | Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin |
title_full_unstemmed | Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin |
title_short | Design and In Vitro Characterization of Buccoadhesive Drug Delivery System of Insulin |
title_sort | design and in vitro characterization of buccoadhesive drug delivery system of insulin |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852063/ https://www.ncbi.nlm.nih.gov/pubmed/20390082 http://dx.doi.org/10.4103/0250-474X.40333 |
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