Isolation of Liver Aldehyde Oxidase Containing Fractions from Different Animals and Determination of Kinetic Parameters for Benzaldehyde

Aldehyde oxidase activity containing fractions from rabbit, guinea pig, rat and mouse livers were obtained by heat treatment and ammonium sulfate precipitation. Aldehyde oxidase activity was observed in rabbit and guinea pig livers, while aldehyde oxidase activity was absent in rat and mouse liver f...

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Detalles Bibliográficos
Autores principales: Kadam, R. S., Iyer, K. R.
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2008
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852067/
https://www.ncbi.nlm.nih.gov/pubmed/20390086
http://dx.doi.org/10.4103/0250-474X.40337
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author Kadam, R. S.
Iyer, K. R.
author_facet Kadam, R. S.
Iyer, K. R.
author_sort Kadam, R. S.
collection PubMed
description Aldehyde oxidase activity containing fractions from rabbit, guinea pig, rat and mouse livers were obtained by heat treatment and ammonium sulfate precipitation. Aldehyde oxidase activity was observed in rabbit and guinea pig livers, while aldehyde oxidase activity was absent in rat and mouse liver fractions. Enzyme kinetic parameters, K(m) and V(max), were determined for the oxidation of benzaldehyde to benzoic acid by rabbit and guinea pig liver fractions, by spectrophotometric method, with potassium ferricyanide as the electron acceptor. The K(m) values obtained for both animal liver fractions were in the range of 10.3-19.1 μM.
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spelling pubmed-28520672010-04-13 Isolation of Liver Aldehyde Oxidase Containing Fractions from Different Animals and Determination of Kinetic Parameters for Benzaldehyde Kadam, R. S. Iyer, K. R. Indian J Pharm Sci Short Communications Aldehyde oxidase activity containing fractions from rabbit, guinea pig, rat and mouse livers were obtained by heat treatment and ammonium sulfate precipitation. Aldehyde oxidase activity was observed in rabbit and guinea pig livers, while aldehyde oxidase activity was absent in rat and mouse liver fractions. Enzyme kinetic parameters, K(m) and V(max), were determined for the oxidation of benzaldehyde to benzoic acid by rabbit and guinea pig liver fractions, by spectrophotometric method, with potassium ferricyanide as the electron acceptor. The K(m) values obtained for both animal liver fractions were in the range of 10.3-19.1 μM. Medknow Publications 2008 /pmc/articles/PMC2852067/ /pubmed/20390086 http://dx.doi.org/10.4103/0250-474X.40337 Text en © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communications
Kadam, R. S.
Iyer, K. R.
Isolation of Liver Aldehyde Oxidase Containing Fractions from Different Animals and Determination of Kinetic Parameters for Benzaldehyde
title Isolation of Liver Aldehyde Oxidase Containing Fractions from Different Animals and Determination of Kinetic Parameters for Benzaldehyde
title_full Isolation of Liver Aldehyde Oxidase Containing Fractions from Different Animals and Determination of Kinetic Parameters for Benzaldehyde
title_fullStr Isolation of Liver Aldehyde Oxidase Containing Fractions from Different Animals and Determination of Kinetic Parameters for Benzaldehyde
title_full_unstemmed Isolation of Liver Aldehyde Oxidase Containing Fractions from Different Animals and Determination of Kinetic Parameters for Benzaldehyde
title_short Isolation of Liver Aldehyde Oxidase Containing Fractions from Different Animals and Determination of Kinetic Parameters for Benzaldehyde
title_sort isolation of liver aldehyde oxidase containing fractions from different animals and determination of kinetic parameters for benzaldehyde
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852067/
https://www.ncbi.nlm.nih.gov/pubmed/20390086
http://dx.doi.org/10.4103/0250-474X.40337
work_keys_str_mv AT kadamrs isolationofliveraldehydeoxidasecontainingfractionsfromdifferentanimalsanddeterminationofkineticparametersforbenzaldehyde
AT iyerkr isolationofliveraldehydeoxidasecontainingfractionsfromdifferentanimalsanddeterminationofkineticparametersforbenzaldehyde

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