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The magic triangle goes MAD: experimental phasing with a bromine derivative

Experimental phasing is an essential technique for the solution of macromolecular structures. Since many heavy-atom ion soaks suffer from nonspecific binding, a novel class of compounds has been developed that combines heavy atoms with functional groups for binding to proteins. The phasing tool 5-am...

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Detalles Bibliográficos
Autores principales: Beck, Tobias, Gruene, Tim, Sheldrick, George M.
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852301/
https://www.ncbi.nlm.nih.gov/pubmed/20382990
http://dx.doi.org/10.1107/S0907444909051609
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author Beck, Tobias
Gruene, Tim
Sheldrick, George M.
author_facet Beck, Tobias
Gruene, Tim
Sheldrick, George M.
author_sort Beck, Tobias
collection PubMed
description Experimental phasing is an essential technique for the solution of macromolecular structures. Since many heavy-atom ion soaks suffer from nonspecific binding, a novel class of compounds has been developed that combines heavy atoms with functional groups for binding to proteins. The phasing tool 5-amino-2,4,6-tribromoisophthalic acid (B3C) contains three functional groups (two carboxylate groups and one amino group) that interact with proteins via hydrogen bonds. Three Br atoms suitable for anomalous dispersion phasing are arranged in an equilateral triangle and are thus readily identified in the heavy-atom substructure. B3C was incorporated into proteinase K and a multiwavelength anomalous dispersion (MAD) experiment at the Br K edge was successfully carried out. Radiation damage to the bromine–carbon bond was investigated. A comparison with the phasing tool I3C that contains three I atoms for single-wavelength anomalous dispersion (SAD) phasing was also carried out.
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spelling pubmed-28523012010-04-13 The magic triangle goes MAD: experimental phasing with a bromine derivative Beck, Tobias Gruene, Tim Sheldrick, George M. Acta Crystallogr D Biol Crystallogr Research Papers Experimental phasing is an essential technique for the solution of macromolecular structures. Since many heavy-atom ion soaks suffer from nonspecific binding, a novel class of compounds has been developed that combines heavy atoms with functional groups for binding to proteins. The phasing tool 5-amino-2,4,6-tribromoisophthalic acid (B3C) contains three functional groups (two carboxylate groups and one amino group) that interact with proteins via hydrogen bonds. Three Br atoms suitable for anomalous dispersion phasing are arranged in an equilateral triangle and are thus readily identified in the heavy-atom substructure. B3C was incorporated into proteinase K and a multiwavelength anomalous dispersion (MAD) experiment at the Br K edge was successfully carried out. Radiation damage to the bromine–carbon bond was investigated. A comparison with the phasing tool I3C that contains three I atoms for single-wavelength anomalous dispersion (SAD) phasing was also carried out. International Union of Crystallography 2010-03-24 /pmc/articles/PMC2852301/ /pubmed/20382990 http://dx.doi.org/10.1107/S0907444909051609 Text en © Beck et al. 2010 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Beck, Tobias
Gruene, Tim
Sheldrick, George M.
The magic triangle goes MAD: experimental phasing with a bromine derivative
title The magic triangle goes MAD: experimental phasing with a bromine derivative
title_full The magic triangle goes MAD: experimental phasing with a bromine derivative
title_fullStr The magic triangle goes MAD: experimental phasing with a bromine derivative
title_full_unstemmed The magic triangle goes MAD: experimental phasing with a bromine derivative
title_short The magic triangle goes MAD: experimental phasing with a bromine derivative
title_sort magic triangle goes mad: experimental phasing with a bromine derivative
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852301/
https://www.ncbi.nlm.nih.gov/pubmed/20382990
http://dx.doi.org/10.1107/S0907444909051609
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