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A tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions
Strigolactones are considered a new group of plant hormones. Their role as modulators of plant growth and signalling molecules for plant interactions first became evident in Arabidopsis, pea, and rice mutants that were flawed in strigolactone production, release, or perception. The first evidence in...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852664/ https://www.ncbi.nlm.nih.gov/pubmed/20194924 http://dx.doi.org/10.1093/jxb/erq041 |
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author | Koltai, Hinanit LekKala, Sivarama P. Bhattacharya, Chaitali Mayzlish-Gati, Einav Resnick, Nathalie Wininger, Smadar Dor, Evgenya Yoneyama, Kaori Yoneyama, Koichi Hershenhorn, Joseph Joel, Daniel M. Kapulnik, Yoram |
author_facet | Koltai, Hinanit LekKala, Sivarama P. Bhattacharya, Chaitali Mayzlish-Gati, Einav Resnick, Nathalie Wininger, Smadar Dor, Evgenya Yoneyama, Kaori Yoneyama, Koichi Hershenhorn, Joseph Joel, Daniel M. Kapulnik, Yoram |
author_sort | Koltai, Hinanit |
collection | PubMed |
description | Strigolactones are considered a new group of plant hormones. Their role as modulators of plant growth and signalling molecules for plant interactions first became evident in Arabidopsis, pea, and rice mutants that were flawed in strigolactone production, release, or perception. The first evidence in tomato (Solanum lycopersicon) of strigolactone deficiency is presented here. Sl-ORT1, previously identified as resistant to the parasitic plant Orobanche, had lower levels of arbuscular mycorrhizal fungus (Glomus intraradices) colonization, possibly as a result of its reduced ability to induce mycorrhizal hyphal branching. Biochemical analysis of mutant root extracts suggested that it produces only minute amounts of two of the tomato strigolactones: solanacol and didehydro-orobanchol. Accordingly, the transcription level of a key enzyme (CCD7) putatively involved in strigolactone synthesis in tomato was reduced in Sl-ORT1 compared with the wild type (WT). Sl-ORT1 shoots exhibited increased lateral shoot branching, whereas exogenous application of the synthetic strigolactone GR24 to the mutant restored the WT phenotype by reducing the number of lateral branches. Reduced lateral shoot branching was also evident in grafted plants which included a WT interstock, which was grafted between the mutant rootstock and the scion. In roots of these grafted plants, the CCD7 transcription level was not significantly induced, nor was mycorrhizal sensitivity restored. Hence, WT-interstock grafting, which restores mutant shoot morphology to WT, does not restore mutant root properties to WT. Characterization of the first tomato strigolactone-deficient mutant supports the putative general role of strigolactones as messengers of suppression of lateral shoot branching in a diversity of plant species. |
format | Text |
id | pubmed-2852664 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-28526642010-04-13 A tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions Koltai, Hinanit LekKala, Sivarama P. Bhattacharya, Chaitali Mayzlish-Gati, Einav Resnick, Nathalie Wininger, Smadar Dor, Evgenya Yoneyama, Kaori Yoneyama, Koichi Hershenhorn, Joseph Joel, Daniel M. Kapulnik, Yoram J Exp Bot Research Papers Strigolactones are considered a new group of plant hormones. Their role as modulators of plant growth and signalling molecules for plant interactions first became evident in Arabidopsis, pea, and rice mutants that were flawed in strigolactone production, release, or perception. The first evidence in tomato (Solanum lycopersicon) of strigolactone deficiency is presented here. Sl-ORT1, previously identified as resistant to the parasitic plant Orobanche, had lower levels of arbuscular mycorrhizal fungus (Glomus intraradices) colonization, possibly as a result of its reduced ability to induce mycorrhizal hyphal branching. Biochemical analysis of mutant root extracts suggested that it produces only minute amounts of two of the tomato strigolactones: solanacol and didehydro-orobanchol. Accordingly, the transcription level of a key enzyme (CCD7) putatively involved in strigolactone synthesis in tomato was reduced in Sl-ORT1 compared with the wild type (WT). Sl-ORT1 shoots exhibited increased lateral shoot branching, whereas exogenous application of the synthetic strigolactone GR24 to the mutant restored the WT phenotype by reducing the number of lateral branches. Reduced lateral shoot branching was also evident in grafted plants which included a WT interstock, which was grafted between the mutant rootstock and the scion. In roots of these grafted plants, the CCD7 transcription level was not significantly induced, nor was mycorrhizal sensitivity restored. Hence, WT-interstock grafting, which restores mutant shoot morphology to WT, does not restore mutant root properties to WT. Characterization of the first tomato strigolactone-deficient mutant supports the putative general role of strigolactones as messengers of suppression of lateral shoot branching in a diversity of plant species. Oxford University Press 2010-06 2010-03-01 /pmc/articles/PMC2852664/ /pubmed/20194924 http://dx.doi.org/10.1093/jxb/erq041 Text en © 2010 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details) |
spellingShingle | Research Papers Koltai, Hinanit LekKala, Sivarama P. Bhattacharya, Chaitali Mayzlish-Gati, Einav Resnick, Nathalie Wininger, Smadar Dor, Evgenya Yoneyama, Kaori Yoneyama, Koichi Hershenhorn, Joseph Joel, Daniel M. Kapulnik, Yoram A tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions |
title | A tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions |
title_full | A tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions |
title_fullStr | A tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions |
title_full_unstemmed | A tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions |
title_short | A tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions |
title_sort | tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852664/ https://www.ncbi.nlm.nih.gov/pubmed/20194924 http://dx.doi.org/10.1093/jxb/erq041 |
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