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Efficient integration of transgenes into a defined locus in human embryonic stem cells

Random integration is one of the more straightforward methods to introduce a transgene into human embryonic stem (ES) cells. However, random integration may result in transgene silencing and altered cell phenotype due to insertional mutagenesis in undefined gene regions. Moreover, reliability of dat...

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Detalles Bibliográficos
Autores principales:	Sakurai, Kenji, Shimoji, Miho, Tahimic, Candice G. T., Aiba, Kazuhiro, Kawase, Eihachiro, Hasegawa, Kouichi, Amagai, Yuji, Suemori, Hirofumi, Nakatsuji, Norio
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2010
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2853137/ https://www.ncbi.nlm.nih.gov/pubmed/20071742 http://dx.doi.org/10.1093/nar/gkp1234

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