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MicroRNA control of podosome formation in vascular smooth muscle cells in vivo and in vitro
Smooth muscle cell (SMC) plasticity plays an important role during development and in vascular pathologies such as atherosclerosis and restenosis. It was recently shown that down-regulation of microRNA (miR)-143 and -145, which are coexpressed from a single promoter, regulates the switch from contra...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2854384/ https://www.ncbi.nlm.nih.gov/pubmed/20351064 http://dx.doi.org/10.1083/jcb.200912096 |
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author | Quintavalle, Manuela Elia, Leonardo Condorelli, Gianluigi Courtneidge, Sara A. |
author_facet | Quintavalle, Manuela Elia, Leonardo Condorelli, Gianluigi Courtneidge, Sara A. |
author_sort | Quintavalle, Manuela |
collection | PubMed |
description | Smooth muscle cell (SMC) plasticity plays an important role during development and in vascular pathologies such as atherosclerosis and restenosis. It was recently shown that down-regulation of microRNA (miR)-143 and -145, which are coexpressed from a single promoter, regulates the switch from contractile to synthetic phenotype, allowing SMCs to migrate and proliferate. We show in this study that loss of miR-143/145 in vitro and in vivo results in the formation of podosomes, which are actin-rich membrane protrusions involved in the migration of several cell types, including SMCs. We further show that platelet-derived growth factor (PDGF) mediates podosome formation in SMCs through the regulation of miR-143/145 expression via a pathway involving Src and p53. Moreover, we identify key podosome regulators as targets of miR-143 (PDGF receptor α and protein kinase C ε) and miR-145 (fascin). Thus, dysregulation of the miR-143 and -145 genes is causally involved in the aberrant SMC plasticity encountered during vascular disease, in part through the up-regulation of an autoregulatory loop that promotes podosome formation. |
format | Text |
id | pubmed-2854384 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-28543842010-10-05 MicroRNA control of podosome formation in vascular smooth muscle cells in vivo and in vitro Quintavalle, Manuela Elia, Leonardo Condorelli, Gianluigi Courtneidge, Sara A. J Cell Biol Research Articles Smooth muscle cell (SMC) plasticity plays an important role during development and in vascular pathologies such as atherosclerosis and restenosis. It was recently shown that down-regulation of microRNA (miR)-143 and -145, which are coexpressed from a single promoter, regulates the switch from contractile to synthetic phenotype, allowing SMCs to migrate and proliferate. We show in this study that loss of miR-143/145 in vitro and in vivo results in the formation of podosomes, which are actin-rich membrane protrusions involved in the migration of several cell types, including SMCs. We further show that platelet-derived growth factor (PDGF) mediates podosome formation in SMCs through the regulation of miR-143/145 expression via a pathway involving Src and p53. Moreover, we identify key podosome regulators as targets of miR-143 (PDGF receptor α and protein kinase C ε) and miR-145 (fascin). Thus, dysregulation of the miR-143 and -145 genes is causally involved in the aberrant SMC plasticity encountered during vascular disease, in part through the up-regulation of an autoregulatory loop that promotes podosome formation. The Rockefeller University Press 2010-04-05 /pmc/articles/PMC2854384/ /pubmed/20351064 http://dx.doi.org/10.1083/jcb.200912096 Text en © 2010 Quintavalle et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Quintavalle, Manuela Elia, Leonardo Condorelli, Gianluigi Courtneidge, Sara A. MicroRNA control of podosome formation in vascular smooth muscle cells in vivo and in vitro |
title | MicroRNA control of podosome formation in vascular smooth muscle cells in vivo and in vitro |
title_full | MicroRNA control of podosome formation in vascular smooth muscle cells in vivo and in vitro |
title_fullStr | MicroRNA control of podosome formation in vascular smooth muscle cells in vivo and in vitro |
title_full_unstemmed | MicroRNA control of podosome formation in vascular smooth muscle cells in vivo and in vitro |
title_short | MicroRNA control of podosome formation in vascular smooth muscle cells in vivo and in vitro |
title_sort | microrna control of podosome formation in vascular smooth muscle cells in vivo and in vitro |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2854384/ https://www.ncbi.nlm.nih.gov/pubmed/20351064 http://dx.doi.org/10.1083/jcb.200912096 |
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