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A cis-Regulatory Signature for Chordate Anterior Neuroectodermal Genes
One of the striking findings of comparative developmental genetics was that expression patterns of core transcription factors are extraordinarily conserved in bilaterians. However, it remains unclear whether cis-regulatory elements of their target genes also exhibit common signatures associated with...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855326/ https://www.ncbi.nlm.nih.gov/pubmed/20419150 http://dx.doi.org/10.1371/journal.pgen.1000912 |
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author | Haeussler, Maximilian Jaszczyszyn, Yan Christiaen, Lionel Joly, Jean-Stéphane |
author_facet | Haeussler, Maximilian Jaszczyszyn, Yan Christiaen, Lionel Joly, Jean-Stéphane |
author_sort | Haeussler, Maximilian |
collection | PubMed |
description | One of the striking findings of comparative developmental genetics was that expression patterns of core transcription factors are extraordinarily conserved in bilaterians. However, it remains unclear whether cis-regulatory elements of their target genes also exhibit common signatures associated with conserved embryonic fields. To address this question, we focused on genes that are active in the anterior neuroectoderm and non-neural ectoderm of the ascidian Ciona intestinalis. Following the dissection of a prototypic anterior placodal enhancer, we searched all genomic conserved non-coding elements for duplicated motifs around genes showing anterior neuroectodermal expression. Strikingly, we identified an over-represented pentamer motif corresponding to the binding site of the homeodomain protein OTX, which plays a pivotal role in the anterior development of all bilaterian species. Using an in vivo reporter gene assay, we observed that 10 of 23 candidate cis-regulatory elements containing duplicated OTX motifs are active in the anterior neuroectoderm, thus showing that this cis-regulatory signature is predictive of neuroectodermal enhancers. These results show that a common cis-regulatory signature corresponding to K50-Paired homeodomain transcription factors is found in non-coding sequences flanking anterior neuroectodermal genes in chordate embryos. Thus, field-specific selector genes impose architectural constraints in the form of combinations of short tags on their target enhancers. This could account for the strong evolutionary conservation of the regulatory elements controlling field-specific selector genes responsible for body plan formation. |
format | Text |
id | pubmed-2855326 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28553262010-04-23 A cis-Regulatory Signature for Chordate Anterior Neuroectodermal Genes Haeussler, Maximilian Jaszczyszyn, Yan Christiaen, Lionel Joly, Jean-Stéphane PLoS Genet Research Article One of the striking findings of comparative developmental genetics was that expression patterns of core transcription factors are extraordinarily conserved in bilaterians. However, it remains unclear whether cis-regulatory elements of their target genes also exhibit common signatures associated with conserved embryonic fields. To address this question, we focused on genes that are active in the anterior neuroectoderm and non-neural ectoderm of the ascidian Ciona intestinalis. Following the dissection of a prototypic anterior placodal enhancer, we searched all genomic conserved non-coding elements for duplicated motifs around genes showing anterior neuroectodermal expression. Strikingly, we identified an over-represented pentamer motif corresponding to the binding site of the homeodomain protein OTX, which plays a pivotal role in the anterior development of all bilaterian species. Using an in vivo reporter gene assay, we observed that 10 of 23 candidate cis-regulatory elements containing duplicated OTX motifs are active in the anterior neuroectoderm, thus showing that this cis-regulatory signature is predictive of neuroectodermal enhancers. These results show that a common cis-regulatory signature corresponding to K50-Paired homeodomain transcription factors is found in non-coding sequences flanking anterior neuroectodermal genes in chordate embryos. Thus, field-specific selector genes impose architectural constraints in the form of combinations of short tags on their target enhancers. This could account for the strong evolutionary conservation of the regulatory elements controlling field-specific selector genes responsible for body plan formation. Public Library of Science 2010-04-15 /pmc/articles/PMC2855326/ /pubmed/20419150 http://dx.doi.org/10.1371/journal.pgen.1000912 Text en Haeussler et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Haeussler, Maximilian Jaszczyszyn, Yan Christiaen, Lionel Joly, Jean-Stéphane A cis-Regulatory Signature for Chordate Anterior Neuroectodermal Genes |
title | A cis-Regulatory Signature for Chordate Anterior Neuroectodermal Genes |
title_full | A cis-Regulatory Signature for Chordate Anterior Neuroectodermal Genes |
title_fullStr | A cis-Regulatory Signature for Chordate Anterior Neuroectodermal Genes |
title_full_unstemmed | A cis-Regulatory Signature for Chordate Anterior Neuroectodermal Genes |
title_short | A cis-Regulatory Signature for Chordate Anterior Neuroectodermal Genes |
title_sort | cis-regulatory signature for chordate anterior neuroectodermal genes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855326/ https://www.ncbi.nlm.nih.gov/pubmed/20419150 http://dx.doi.org/10.1371/journal.pgen.1000912 |
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