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Derivation of 30 human embryonic stem cell lines—improving the quality

We have derived 30 human embryonic stem cell lines from supernumerary blastocysts in our laboratory. During the derivation process, we have studied new and safe method to establish good quality lines. All our human embryonic stem cell lines have been derived using human foreskin fibroblasts as feede...

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Detalles Bibliográficos
Autores principales: Ström, Susanne, Holm, Frida, Bergström, Rosita, Strömberg, Anne-Marie, Hovatta, Outi
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855803/
https://www.ncbi.nlm.nih.gov/pubmed/20198446
http://dx.doi.org/10.1007/s11626-010-9308-0
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author Ström, Susanne
Holm, Frida
Bergström, Rosita
Strömberg, Anne-Marie
Hovatta, Outi
author_facet Ström, Susanne
Holm, Frida
Bergström, Rosita
Strömberg, Anne-Marie
Hovatta, Outi
author_sort Ström, Susanne
collection PubMed
description We have derived 30 human embryonic stem cell lines from supernumerary blastocysts in our laboratory. During the derivation process, we have studied new and safe method to establish good quality lines. All our human embryonic stem cell lines have been derived using human foreskin fibroblasts as feeder cells. The 26 more recent lines were derived in a medium containing serum replacement instead of fetal calf serum. Mechanical isolation of the inner cell mass using flexible metal needles was used in deriving the 10 latest lines. The lines are karyotypically normal, but culture adaptation in two lines has been observed. Our human embryonic stem cell lines are banked, and they are available for researchers.
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spelling pubmed-28558032010-04-25 Derivation of 30 human embryonic stem cell lines—improving the quality Ström, Susanne Holm, Frida Bergström, Rosita Strömberg, Anne-Marie Hovatta, Outi In Vitro Cell Dev Biol Anim Article We have derived 30 human embryonic stem cell lines from supernumerary blastocysts in our laboratory. During the derivation process, we have studied new and safe method to establish good quality lines. All our human embryonic stem cell lines have been derived using human foreskin fibroblasts as feeder cells. The 26 more recent lines were derived in a medium containing serum replacement instead of fetal calf serum. Mechanical isolation of the inner cell mass using flexible metal needles was used in deriving the 10 latest lines. The lines are karyotypically normal, but culture adaptation in two lines has been observed. Our human embryonic stem cell lines are banked, and they are available for researchers. Springer-Verlag 2010-03-03 2010 /pmc/articles/PMC2855803/ /pubmed/20198446 http://dx.doi.org/10.1007/s11626-010-9308-0 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Ström, Susanne
Holm, Frida
Bergström, Rosita
Strömberg, Anne-Marie
Hovatta, Outi
Derivation of 30 human embryonic stem cell lines—improving the quality
title Derivation of 30 human embryonic stem cell lines—improving the quality
title_full Derivation of 30 human embryonic stem cell lines—improving the quality
title_fullStr Derivation of 30 human embryonic stem cell lines—improving the quality
title_full_unstemmed Derivation of 30 human embryonic stem cell lines—improving the quality
title_short Derivation of 30 human embryonic stem cell lines—improving the quality
title_sort derivation of 30 human embryonic stem cell lines—improving the quality
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855803/
https://www.ncbi.nlm.nih.gov/pubmed/20198446
http://dx.doi.org/10.1007/s11626-010-9308-0
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