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Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions
Lymph node stromal cells (LNSCs) can induce potent, antigen-specific T cell tolerance under steady-state conditions. Although expression of various peripheral tissue–restricted antigens (PTAs) and presentation to naive CD8(+) T cells has been demonstrated, the stromal subsets responsible have not be...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2856033/ https://www.ncbi.nlm.nih.gov/pubmed/20308362 http://dx.doi.org/10.1084/jem.20092642 |
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author | Fletcher, Anne L. Lukacs-Kornek, Veronika Reynoso, Erika D. Pinner, Sophie E. Bellemare-Pelletier, Angelique Curry, Mark S. Collier, Ai-Ris Boyd, Richard L. Turley, Shannon J. |
author_facet | Fletcher, Anne L. Lukacs-Kornek, Veronika Reynoso, Erika D. Pinner, Sophie E. Bellemare-Pelletier, Angelique Curry, Mark S. Collier, Ai-Ris Boyd, Richard L. Turley, Shannon J. |
author_sort | Fletcher, Anne L. |
collection | PubMed |
description | Lymph node stromal cells (LNSCs) can induce potent, antigen-specific T cell tolerance under steady-state conditions. Although expression of various peripheral tissue–restricted antigens (PTAs) and presentation to naive CD8(+) T cells has been demonstrated, the stromal subsets responsible have not been identified. We report that fibroblastic reticular cells (FRCs), which reside in the T cell zone of the LN, ectopically express and directly present a model PTA to naive T cells, inducing their proliferation. However, we found that no single LNSC subset was responsible for PTA expression; rather, each subset had its own characteristic antigen display. Studies to date have concentrated on PTA presentation under steady-state conditions; however, because LNs are frequently inflammatory sites, we assessed whether inflammation altered stromal cell–T cell interactions. Strikingly, FRCs showed reduced stimulation of T cells after Toll-like receptor 3 ligation. We also characterize an LNSC subset expressing the highest levels of autoimmune regulator, which responds potently to bystander inflammation by up-regulating PTA expression. Collectively, these data show that diverse stromal cell types have evolved to constitutively express PTAs, and that exposure to viral products alters the interaction between T cells and LNSCs. |
format | Text |
id | pubmed-2856033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-28560332010-10-12 Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions Fletcher, Anne L. Lukacs-Kornek, Veronika Reynoso, Erika D. Pinner, Sophie E. Bellemare-Pelletier, Angelique Curry, Mark S. Collier, Ai-Ris Boyd, Richard L. Turley, Shannon J. J Exp Med Brief Definitive Report Lymph node stromal cells (LNSCs) can induce potent, antigen-specific T cell tolerance under steady-state conditions. Although expression of various peripheral tissue–restricted antigens (PTAs) and presentation to naive CD8(+) T cells has been demonstrated, the stromal subsets responsible have not been identified. We report that fibroblastic reticular cells (FRCs), which reside in the T cell zone of the LN, ectopically express and directly present a model PTA to naive T cells, inducing their proliferation. However, we found that no single LNSC subset was responsible for PTA expression; rather, each subset had its own characteristic antigen display. Studies to date have concentrated on PTA presentation under steady-state conditions; however, because LNs are frequently inflammatory sites, we assessed whether inflammation altered stromal cell–T cell interactions. Strikingly, FRCs showed reduced stimulation of T cells after Toll-like receptor 3 ligation. We also characterize an LNSC subset expressing the highest levels of autoimmune regulator, which responds potently to bystander inflammation by up-regulating PTA expression. Collectively, these data show that diverse stromal cell types have evolved to constitutively express PTAs, and that exposure to viral products alters the interaction between T cells and LNSCs. The Rockefeller University Press 2010-04-12 /pmc/articles/PMC2856033/ /pubmed/20308362 http://dx.doi.org/10.1084/jem.20092642 Text en © 2010 Fletcher et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Brief Definitive Report Fletcher, Anne L. Lukacs-Kornek, Veronika Reynoso, Erika D. Pinner, Sophie E. Bellemare-Pelletier, Angelique Curry, Mark S. Collier, Ai-Ris Boyd, Richard L. Turley, Shannon J. Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions |
title | Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions |
title_full | Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions |
title_fullStr | Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions |
title_full_unstemmed | Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions |
title_short | Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions |
title_sort | lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions |
topic | Brief Definitive Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2856033/ https://www.ncbi.nlm.nih.gov/pubmed/20308362 http://dx.doi.org/10.1084/jem.20092642 |
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