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Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection
Host immune responses, including the characteristic influx of neutrophils, against Neisseria gonorrhoeae are poorly understood; adaptive immunity is minimal and nonprotective. We hypothesize that N. gonorrhoeae selectively elicits Th17-dependent responses which recruit innate defense mechanisms incl...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2857675/ https://www.ncbi.nlm.nih.gov/pubmed/20107432 http://dx.doi.org/10.1038/mi.2009.139 |
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author | Feinen, Brandon Jerse, Ann E. Gaffen, Sarah L. Russell, Michael W. |
author_facet | Feinen, Brandon Jerse, Ann E. Gaffen, Sarah L. Russell, Michael W. |
author_sort | Feinen, Brandon |
collection | PubMed |
description | Host immune responses, including the characteristic influx of neutrophils, against Neisseria gonorrhoeae are poorly understood; adaptive immunity is minimal and nonprotective. We hypothesize that N. gonorrhoeae selectively elicits Th17-dependent responses which recruit innate defense mechanisms including neutrophils and antimicrobial proteins that it can resist. We found that N. gonorrhoeae induced production of IL-17 in mouse T cells and of Th17-inducing cytokines in mouse and human antigen-presenting cells in vitro. IL-17 was induced in the iliac lymph nodes in vivo in a female mouse model of genital tract gonococcal infection. Antibody blockade of IL-17 or deletion of the major IL-17 receptor in IL-17RA-knockoutmice led to prolonged infection and diminished neutrophil influx. Genital tract tissue from IL-17RA-knockout mice showed reduced production of neutrophil-attractant chemokines in response to culture with N. gonorrhoeae. These results imply a crucial role for IL-17 and Th17 cells in the immune response to N. gonorrhoeae. |
format | Text |
id | pubmed-2857675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
record_format | MEDLINE/PubMed |
spelling | pubmed-28576752010-11-01 Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection Feinen, Brandon Jerse, Ann E. Gaffen, Sarah L. Russell, Michael W. Mucosal Immunol Article Host immune responses, including the characteristic influx of neutrophils, against Neisseria gonorrhoeae are poorly understood; adaptive immunity is minimal and nonprotective. We hypothesize that N. gonorrhoeae selectively elicits Th17-dependent responses which recruit innate defense mechanisms including neutrophils and antimicrobial proteins that it can resist. We found that N. gonorrhoeae induced production of IL-17 in mouse T cells and of Th17-inducing cytokines in mouse and human antigen-presenting cells in vitro. IL-17 was induced in the iliac lymph nodes in vivo in a female mouse model of genital tract gonococcal infection. Antibody blockade of IL-17 or deletion of the major IL-17 receptor in IL-17RA-knockoutmice led to prolonged infection and diminished neutrophil influx. Genital tract tissue from IL-17RA-knockout mice showed reduced production of neutrophil-attractant chemokines in response to culture with N. gonorrhoeae. These results imply a crucial role for IL-17 and Th17 cells in the immune response to N. gonorrhoeae. 2010-01-27 2010-05 /pmc/articles/PMC2857675/ /pubmed/20107432 http://dx.doi.org/10.1038/mi.2009.139 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Feinen, Brandon Jerse, Ann E. Gaffen, Sarah L. Russell, Michael W. Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection |
title | Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection |
title_full | Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection |
title_fullStr | Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection |
title_full_unstemmed | Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection |
title_short | Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection |
title_sort | critical role of th17 responses in a murine model of neisseria gonorrhoeae genital infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2857675/ https://www.ncbi.nlm.nih.gov/pubmed/20107432 http://dx.doi.org/10.1038/mi.2009.139 |
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