The chemical-in-plug bacterial chemotaxis assay is prone to false positive responses

BACKGROUND: Chemical-in-plug assays are commonly used to study bacterial chemotaxis, sometimes in the absence of stringent controls. RESULTS: We report that non-chemotactic and non-motile mutants in two distinct bacterial species (Shewanella oneidensis and Helicobacter pylori) show apparent zones of...

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Detalles Bibliográficos
Autores principales: Li, Jun, Go, Alvin C, Ward, Mandy J, Ottemann, Karen M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2857857/
https://www.ncbi.nlm.nih.gov/pubmed/20233446
http://dx.doi.org/10.1186/1756-0500-3-77
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author Li, Jun
Go, Alvin C
Ward, Mandy J
Ottemann, Karen M
author_facet Li, Jun
Go, Alvin C
Ward, Mandy J
Ottemann, Karen M
author_sort Li, Jun
collection PubMed
description BACKGROUND: Chemical-in-plug assays are commonly used to study bacterial chemotaxis, sometimes in the absence of stringent controls. RESULTS: We report that non-chemotactic and non-motile mutants in two distinct bacterial species (Shewanella oneidensis and Helicobacter pylori) show apparent zones of accumulation or clearing around test plugs containing potential attractants or repellents, respectively. CONCLUSIONS: Our results suggest that the chemical-in-plug assay should be used with caution, that non-motile or non-chemotactic mutants should be employed as controls, and that results should be confirmed with other types of assays.
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spelling pubmed-28578572010-04-22 The chemical-in-plug bacterial chemotaxis assay is prone to false positive responses Li, Jun Go, Alvin C Ward, Mandy J Ottemann, Karen M BMC Res Notes Research article BACKGROUND: Chemical-in-plug assays are commonly used to study bacterial chemotaxis, sometimes in the absence of stringent controls. RESULTS: We report that non-chemotactic and non-motile mutants in two distinct bacterial species (Shewanella oneidensis and Helicobacter pylori) show apparent zones of accumulation or clearing around test plugs containing potential attractants or repellents, respectively. CONCLUSIONS: Our results suggest that the chemical-in-plug assay should be used with caution, that non-motile or non-chemotactic mutants should be employed as controls, and that results should be confirmed with other types of assays. BioMed Central 2010-03-16 /pmc/articles/PMC2857857/ /pubmed/20233446 http://dx.doi.org/10.1186/1756-0500-3-77 Text en Copyright ©2010 Ward et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Li, Jun
Go, Alvin C
Ward, Mandy J
Ottemann, Karen M
The chemical-in-plug bacterial chemotaxis assay is prone to false positive responses
title The chemical-in-plug bacterial chemotaxis assay is prone to false positive responses
title_full The chemical-in-plug bacterial chemotaxis assay is prone to false positive responses
title_fullStr The chemical-in-plug bacterial chemotaxis assay is prone to false positive responses
title_full_unstemmed The chemical-in-plug bacterial chemotaxis assay is prone to false positive responses
title_short The chemical-in-plug bacterial chemotaxis assay is prone to false positive responses
title_sort chemical-in-plug bacterial chemotaxis assay is prone to false positive responses
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2857857/
https://www.ncbi.nlm.nih.gov/pubmed/20233446
http://dx.doi.org/10.1186/1756-0500-3-77
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