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Antibody Fragment Expression and Purification

Interest in the potential of monoclonal antibodies (mAbs) to serve as therapeutic agents has surged in the past decade with a major emphasis on human viral diseases. There has been much attention in this area directed towards the human immunodeficiency virus type-1 (HIV-1) and promising research dev...

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Autores principales: Dimitrova, Dimana, Choudhry, Vidita, Broder, Christopher C.
Formato: Texto
Lenguaje:English
Publicado: 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858623/
https://www.ncbi.nlm.nih.gov/pubmed/19252844
http://dx.doi.org/10.1007/978-1-59745-554-1_25
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author Dimitrova, Dimana
Choudhry, Vidita
Broder, Christopher C.
author_facet Dimitrova, Dimana
Choudhry, Vidita
Broder, Christopher C.
author_sort Dimitrova, Dimana
collection PubMed
description Interest in the potential of monoclonal antibodies (mAbs) to serve as therapeutic agents has surged in the past decade with a major emphasis on human viral diseases. There has been much attention in this area directed towards the human immunodeficiency virus type-1 (HIV-1) and promising research developments have emerged on the inhibition of HIV-1 infection by mAbs and the identification of several highly conserved neutralizing epitopes. More recently, potent fully-human neutralizing mAbs have been developed against a variety of important human viral disease agents including the paramyxoviruses Hendra virus and Nipah virus, and human or humanized mAbs have been developed against severe acute respiratory syndrome coronavirus (SARS CoV), and West Nile virus, among others. Most of these more recently developed antiviral mAbs have come from the use of antibody phage-display technologies and the implementation of simplified, inexpensive yet efficient methods, for expressing and purifying the initially selected fragment antibodies is of prime importance in further facilitating this area of research.
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spelling pubmed-28586232010-04-22 Antibody Fragment Expression and Purification Dimitrova, Dimana Choudhry, Vidita Broder, Christopher C. Therapeutic Antibodies Article Interest in the potential of monoclonal antibodies (mAbs) to serve as therapeutic agents has surged in the past decade with a major emphasis on human viral diseases. There has been much attention in this area directed towards the human immunodeficiency virus type-1 (HIV-1) and promising research developments have emerged on the inhibition of HIV-1 infection by mAbs and the identification of several highly conserved neutralizing epitopes. More recently, potent fully-human neutralizing mAbs have been developed against a variety of important human viral disease agents including the paramyxoviruses Hendra virus and Nipah virus, and human or humanized mAbs have been developed against severe acute respiratory syndrome coronavirus (SARS CoV), and West Nile virus, among others. Most of these more recently developed antiviral mAbs have come from the use of antibody phage-display technologies and the implementation of simplified, inexpensive yet efficient methods, for expressing and purifying the initially selected fragment antibodies is of prime importance in further facilitating this area of research. 2008-09-09 /pmc/articles/PMC2858623/ /pubmed/19252844 http://dx.doi.org/10.1007/978-1-59745-554-1_25 Text en © Humana Press, a part of Springer Science+Business Media, LLC 2009 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Dimitrova, Dimana
Choudhry, Vidita
Broder, Christopher C.
Antibody Fragment Expression and Purification
title Antibody Fragment Expression and Purification
title_full Antibody Fragment Expression and Purification
title_fullStr Antibody Fragment Expression and Purification
title_full_unstemmed Antibody Fragment Expression and Purification
title_short Antibody Fragment Expression and Purification
title_sort antibody fragment expression and purification
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858623/
https://www.ncbi.nlm.nih.gov/pubmed/19252844
http://dx.doi.org/10.1007/978-1-59745-554-1_25
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