Cargando…

Oligonucleotide microarray for the identification of potential mycotoxigenic fungi

BACKGROUND: Mycotoxins are secondary metabolites which are produced by numerous fungi and pose a continuous challenge to the safety and quality of food commodities in South Africa. These toxins have toxicologically relevant effects on humans and animals that eat contaminated foods. In this study, a...

Descripción completa

Detalles Bibliográficos
Autores principales: Lezar, Sabine, Barros, Eugenia
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858739/
https://www.ncbi.nlm.nih.gov/pubmed/20307326
http://dx.doi.org/10.1186/1471-2180-10-87
_version_ 1782180446828756992
author Lezar, Sabine
Barros, Eugenia
author_facet Lezar, Sabine
Barros, Eugenia
author_sort Lezar, Sabine
collection PubMed
description BACKGROUND: Mycotoxins are secondary metabolites which are produced by numerous fungi and pose a continuous challenge to the safety and quality of food commodities in South Africa. These toxins have toxicologically relevant effects on humans and animals that eat contaminated foods. In this study, a diagnostic DNA microarray was developed for the identification of the most common food-borne fungi, as well as the genes leading to toxin production. RESULTS: A total of 40 potentially mycotoxigenic fungi isolated from different food commodities, as well as the genes that are involved in the mycotoxin synthetic pathways, were analyzed. For fungal identification, oligonucleotide probes were designed by exploiting the sequence variations of the elongation factor 1-alpha (EF-1 α) coding regions and the internal transcribed spacer (ITS) regions of the rRNA gene cassette. For the detection of fungi able to produce mycotoxins, oligonucleotide probes directed towards genes leading to toxin production from different fungal strains were identified in data available in the public domain. The probes selected for fungal identification and the probes specific for toxin producing genes were spotted onto microarray slides. CONCLUSIONS: The diagnostic microarray developed can be used to identify single pure strains or cultures of potentially mycotoxigenic fungi as well as genes leading to toxin production in both laboratory samples and maize-derived foods offering an interesting potential for microbiological laboratories.
format Text
id pubmed-2858739
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-28587392010-04-23 Oligonucleotide microarray for the identification of potential mycotoxigenic fungi Lezar, Sabine Barros, Eugenia BMC Microbiol Research article BACKGROUND: Mycotoxins are secondary metabolites which are produced by numerous fungi and pose a continuous challenge to the safety and quality of food commodities in South Africa. These toxins have toxicologically relevant effects on humans and animals that eat contaminated foods. In this study, a diagnostic DNA microarray was developed for the identification of the most common food-borne fungi, as well as the genes leading to toxin production. RESULTS: A total of 40 potentially mycotoxigenic fungi isolated from different food commodities, as well as the genes that are involved in the mycotoxin synthetic pathways, were analyzed. For fungal identification, oligonucleotide probes were designed by exploiting the sequence variations of the elongation factor 1-alpha (EF-1 α) coding regions and the internal transcribed spacer (ITS) regions of the rRNA gene cassette. For the detection of fungi able to produce mycotoxins, oligonucleotide probes directed towards genes leading to toxin production from different fungal strains were identified in data available in the public domain. The probes selected for fungal identification and the probes specific for toxin producing genes were spotted onto microarray slides. CONCLUSIONS: The diagnostic microarray developed can be used to identify single pure strains or cultures of potentially mycotoxigenic fungi as well as genes leading to toxin production in both laboratory samples and maize-derived foods offering an interesting potential for microbiological laboratories. BioMed Central 2010-03-23 /pmc/articles/PMC2858739/ /pubmed/20307326 http://dx.doi.org/10.1186/1471-2180-10-87 Text en Copyright ©2010 Lezar and Barros; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Lezar, Sabine
Barros, Eugenia
Oligonucleotide microarray for the identification of potential mycotoxigenic fungi
title Oligonucleotide microarray for the identification of potential mycotoxigenic fungi
title_full Oligonucleotide microarray for the identification of potential mycotoxigenic fungi
title_fullStr Oligonucleotide microarray for the identification of potential mycotoxigenic fungi
title_full_unstemmed Oligonucleotide microarray for the identification of potential mycotoxigenic fungi
title_short Oligonucleotide microarray for the identification of potential mycotoxigenic fungi
title_sort oligonucleotide microarray for the identification of potential mycotoxigenic fungi
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858739/
https://www.ncbi.nlm.nih.gov/pubmed/20307326
http://dx.doi.org/10.1186/1471-2180-10-87
work_keys_str_mv AT lezarsabine oligonucleotidemicroarrayfortheidentificationofpotentialmycotoxigenicfungi
AT barroseugenia oligonucleotidemicroarrayfortheidentificationofpotentialmycotoxigenicfungi