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K30, H150, and H168 Are Essential Residues for Coordinating Pyridoxal 5′-Phosphate of O-Acetylserine Sulfhydrylase from Acidithiobacillus ferrooxidans

O-acetylserine sulfhydrylase (OASS) is a key enzyme involved in the pathway of the cysteine biosynthesis. The gene of OASS from Acidithiobacillus ferrooxidans ATCC 23270 was cloned and expressed in E. coli, the soluble protein was purified by one-step affinity chromatography to apparent homogeneity....

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Detalles Bibliográficos
Autores principales:	Zheng, Chunli, Nie, Li, Qian, Lin, Wang, Zhilou, Liu, Guizhen, Liu, Jianshe
Formato:	Texto
Lenguaje:	English
Publicado:	Springer-Verlag 2009
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2859159/ https://www.ncbi.nlm.nih.gov/pubmed/20033172 http://dx.doi.org/10.1007/s00284-009-9565-x

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