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Activation of σ(28)-dependent transcription in Escherichia coli by the cyclic AMP receptor protein requires an unusual promoter organization

The Escherichia coli aer regulatory region contains a single promoter that is recognized by RNA polymerase containing the flagellar sigma factor, σ(28). Expression from this promoter is dependent on direct activation by the cyclic AMP receptor protein, which binds to a target centred 49.5 base pairs...

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Detalles Bibliográficos
Autores principales: Hollands, Kerry, Lee, David J, Lloyd, Georgina S, Busby, Stephen J W
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2859248/
https://www.ncbi.nlm.nih.gov/pubmed/19843224
http://dx.doi.org/10.1111/j.1365-2958.2009.06913.x
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author Hollands, Kerry
Lee, David J
Lloyd, Georgina S
Busby, Stephen J W
author_facet Hollands, Kerry
Lee, David J
Lloyd, Georgina S
Busby, Stephen J W
author_sort Hollands, Kerry
collection PubMed
description The Escherichia coli aer regulatory region contains a single promoter that is recognized by RNA polymerase containing the flagellar sigma factor, σ(28). Expression from this promoter is dependent on direct activation by the cyclic AMP receptor protein, which binds to a target centred 49.5 base pairs upstream from the transcript start. Activator-dependent transcription from the aer promoter was reconstituted in vitro, and a tethered inorganic nuclease was used to find the position of the C-terminal domains of the RNA polymerase α subunits in transcriptionally competent open complexes. We report that the ternary activator–RNA polymerase–aer promoter open complex is organized differently from complexes at previously characterized promoters. Among other E. coli promoters recognized by RNA polymerase containing σ(28), only the trg promoter is activated directly by the cyclic AMP receptor protein. The organization of the different promoter elements and the activator binding site at the trg promoter is the same as at the aer promoter, suggesting a common activation mechanism.
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spelling pubmed-28592482010-05-07 Activation of σ(28)-dependent transcription in Escherichia coli by the cyclic AMP receptor protein requires an unusual promoter organization Hollands, Kerry Lee, David J Lloyd, Georgina S Busby, Stephen J W Mol Microbiol Research Articles The Escherichia coli aer regulatory region contains a single promoter that is recognized by RNA polymerase containing the flagellar sigma factor, σ(28). Expression from this promoter is dependent on direct activation by the cyclic AMP receptor protein, which binds to a target centred 49.5 base pairs upstream from the transcript start. Activator-dependent transcription from the aer promoter was reconstituted in vitro, and a tethered inorganic nuclease was used to find the position of the C-terminal domains of the RNA polymerase α subunits in transcriptionally competent open complexes. We report that the ternary activator–RNA polymerase–aer promoter open complex is organized differently from complexes at previously characterized promoters. Among other E. coli promoters recognized by RNA polymerase containing σ(28), only the trg promoter is activated directly by the cyclic AMP receptor protein. The organization of the different promoter elements and the activator binding site at the trg promoter is the same as at the aer promoter, suggesting a common activation mechanism. Blackwell Publishing Ltd 2010-03 2009-11-04 /pmc/articles/PMC2859248/ /pubmed/19843224 http://dx.doi.org/10.1111/j.1365-2958.2009.06913.x Text en Journal compilation © 2010 Blackwell Publishing http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Research Articles
Hollands, Kerry
Lee, David J
Lloyd, Georgina S
Busby, Stephen J W
Activation of σ(28)-dependent transcription in Escherichia coli by the cyclic AMP receptor protein requires an unusual promoter organization
title Activation of σ(28)-dependent transcription in Escherichia coli by the cyclic AMP receptor protein requires an unusual promoter organization
title_full Activation of σ(28)-dependent transcription in Escherichia coli by the cyclic AMP receptor protein requires an unusual promoter organization
title_fullStr Activation of σ(28)-dependent transcription in Escherichia coli by the cyclic AMP receptor protein requires an unusual promoter organization
title_full_unstemmed Activation of σ(28)-dependent transcription in Escherichia coli by the cyclic AMP receptor protein requires an unusual promoter organization
title_short Activation of σ(28)-dependent transcription in Escherichia coli by the cyclic AMP receptor protein requires an unusual promoter organization
title_sort activation of σ(28)-dependent transcription in escherichia coli by the cyclic amp receptor protein requires an unusual promoter organization
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2859248/
https://www.ncbi.nlm.nih.gov/pubmed/19843224
http://dx.doi.org/10.1111/j.1365-2958.2009.06913.x
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